IMPLICATIONS OF WATER STRESS-INDUCED CHANGES IN THE LEVELS OF ENDOGENOUS ASCORBIC-ACID AND HYDROGEN-PEROXIDE IN VIGNA SEEDLINGS
IMPLICATIONS OF WATER STRESS-INDUCED CHANGES IN THE LEVELS OF ENDOGENOUS ASCORBIC-ACID AND HYDROGEN-PEROXIDE IN VIGNA SEEDLINGS
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DOI：
10.1111/j.1399-3054.1983.tb04162.x
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发表时间：
1983-01-01
影响因子：
6.4
通讯作者：
CHOUDHURI, MA
中科院分区：
文献类型：
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作者：
MUKHERJEE, SP;CHOUDHURI, MA
V.Catjang Endl.CV。随着水分胁迫程度的增加(-0.5、-1.0、-1.5 Mpa)，PUSA Barsati幼苗的乙醇酸氧化酶活性、H_2O_2和脯氨酸含量基本成比例增加，而过氧化氢酶活性、抗坏血酸和蛋白质含量下降。叶水势(叶.PSI.)相对含水量(RWC)也随胁迫程度的增加而降低。L-半胱氨酸和还原型谷胱甘肽(10-3M)处理降低了叶片的乙醇酸氧化酶活性、H_2O_2含量、抗坏血酸氧化酶活性和脯氨酸含量，也略微改善了胁迫2d的叶片的水分状况。用这些抗氧化剂对非胁迫幼苗进行预处理，效果很小，甚至没有效果。抗氧化剂处理通过调节胁迫组织中内源过氧化氢和抗坏血酸的水平，使植物对水分胁迫具有耐受性。
V. catjang Endl. cv. Pusa Barsati seedlings, subjected to increasing degrees of water stress (-0.5, -1.0, -1.5 MPa [megapascal]), produced an approximately proportional increase in glycolate oxidase activity, H2O2 and proline content but a decrease in catalase activity, ascorbic acid and protein content. Leaf water potential (leaf .psi.) and relative water content (RWC) were also lowered with increasing stress. Pretreatment with L-cysteine and reduced glutathione (10-3 M) decreased glycolate oxidase activity, H2O2 content, ascorbic acid oxidase activity, proline content and also slightly improved the water status of leaves stressed (-1.0 MPa) for 2 days. Pretreatment of non-stressed seedlings with these antioxidants had little or no effect. Treatment with antioxidants makes the plant tolerant against water stress by modulating the endogenous levels of H2O2 and ascorbic acid in stressed tissue.