Polymer-based monolithic columns in capillary format tailored by using controlled in situ polymerization.
Polymer-based monolithic columns in capillary format tailored by using controlled in situ polymerization.
复制标题
DOI：
10.1002/jssc.200800508
复制
发表时间：
2009-02
影响因子：
3.1
通讯作者：
H. Aoki;N. Tanaka;T. Kubo;K. Hosoya
中科院分区：
文献类型：
--
作者：
H. Aoki;N. Tanaka;T. Kubo;K. Hosoya
本综述向读者介绍了我们对用于药物候选物等小分子溶质的高性能聚合物基整体柱的新观点，并阐述了毛细管液相色谱柱的制备。首先，我们简要回顾了聚合物基整体柱的现状，并与硅胶整体柱进行了比较。从概念上强调了具有更高通量（更短分析时间）的液相色谱系统的小型化，以及具有亚微米域尺寸（骨架厚度 + 孔径）的良好渗透性双连续整体结构以实现更高性能。其次，从这些角度描述了我们的柱制备过程，同时介绍了我们专门设计的致孔溶剂，它通过反应诱导相分离来控制整体柱的形态。具体而言，以两种聚合物形式举例说明了整体柱，即一种是由1,3 - 二甲基丙烯酸甘油酯（GDMA）自由基聚合制备的整体柱，另一种是由新引入的多官能环氧和二氨基单体逐步聚合制备的整体柱。两种毛细管形式的整体柱都呈现出良好的双连续结构，在效率（H）和渗透性（D）方面具有良好的兼容性。特别是，环氧基柱表现出优异的分离阻抗，E（=H²/D）。我们结合一个原型有线芯片装置对微液相色谱数据进行了讨论。
This review introduces to the readers our new perspectives of polymer-based monolithic column with a high performance for small solutes such as drug candidates, illustrating the fabrication of LC columns in capillary. First, we briefly reviewed the status quo of polymer-based monolithic columns, comparing with silica monoliths. The miniaturization of LC system with higher throughput (shorter analytical time) was stressed conceptually, along with a fine permeable bicontinuous monolithic structure with submicron domain size (skeletal thickness + pore size) for higher performance. Second, from these perspectives, our column preparation was described, while our specially designed porogenic solvents were introduced as a controller of the monolithic morphology via reaction-induced phase separation. Specifically, monolithic columns were exemplified in two polymer formats, that is, one monolith prepared by free radical polymerization of glycerin 1,3-dimethacrylate, GDMA, and the other prepared by stepwise polymerization of newly introduced multifunctional epoxy and diamino monomers. Both monolithic columns in capillary format demonstrated a fine bicontinuous structure, affording a good compatibility of the efficiency (H) and permeability (D). Especially, the epoxy-based column showed an excellent separation impedance, E (=H(2)/D). Our micro-HPLC data were discussed along with a prototyped wired chip device.