RESTRICTION OF DNA IN YERSINIA-ENTEROCOLITICA DETECTED BY RECIPIENT ABILITY FOR A DEREPRESSED R-FACTOR FROM ESCHERICHIA-COLI
RESTRICTION OF DNA IN YERSINIA-ENTEROCOLITICA DETECTED BY RECIPIENT ABILITY FOR A DEREPRESSED R-FACTOR FROM ESCHERICHIA-COLI
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DOI：
10.1099/00221287-87-2-285
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
COLSON, C
中科院分区：
文献类型：
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作者：
CORNELIS, G;COLSON, C
抑制的R因子RY2drd2以5 × 10−3的频率在膜上小肠结肠炎耶尔森菌的两株菌株之间转移。在相同条件下，从大肠杆菌中转移R因子。小肠结肠炎的发生率仅为7·7 × 10−6。当接收菌株在交配前进行热处理时，这种频率大大增加。最佳育力的热暴露为50至52°C，持续2至3分钟。突变体ofY。从大肠杆菌中分离出感染RY2drd2的小肠结肠炎菌。colior fromY。肠结肠炎发生率相同。这些观察结果有力地提示了DNA限制和修饰系统。小肠结肠炎的原因是它对其他物种质粒的接受能力较低。
A derepressed R factor, RY2drd2, was transferred at a frequency of 5 × 10−3between two strains ofYersinia enterocoliticamated on a membrane. Under the same conditions transfer of this R factor fromEscherichia colitoY. enterocoliticawas observed at a frequency of only 7·7 × 10−6. This frequency was greatly increased when the recipient strain was heat-treated before mating. Heat exposure for optimum fertility was 50 to 52 °C for a period of 2 to 3 min. Mutants ofY. enterocoliticawere isolated which were infected RY2drd2 fromE. colior fromY. enterocoliticaat the same frequency. These observations strongly suggest that a DNA restriction and modification system inY. enterocoliticacauses its low recipient ability for plasmids from other species.