Seasonal variation in environmental DNA detection in sediment and water samples
Seasonal variation in environmental DNA detection in sediment and water samples
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DOI：
10.1371/journal.pone.0191737
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发表时间：
2018-01-19
期刊：
影响因子：
3.7
通讯作者：
Griffiths, Richard A.
中科院分区：
文献类型：
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作者：
Buxton, Andrew S.;Groombridge, Jim J.;Griffiths, Richard A.
使用水生环境DNA（eDNA）来检测物种的存在取决于物种在采样栖息地的季节性活动。eDNA在沉积物中的存留时间可能比在水中的存留时间更长，分析沉积物可能会延长物种评估的季节窗口。以大冠蝾螈为模型，我们比较了从池塘水和池塘沉积物中收集的eDNA样本中检测概率如何在季节变化。水生和沉积eDNA的检测全年变化，在夏季（7月）达到高峰，其最低点在冬季（1月）：在所有季节，从水的eDNA的检测概率超过了从沉积物。eDNA的检测概率也各不相同的研究领域，并根据大冠蝾螈栖息地的适宜性和沉积物类型。由于水生和沉积eDNA显示出相同的季节性波动，在这两种样本类型中观察到的模式可能反映了目标物种的当前或近期存在。然而，鉴于在秋季和冬季发现的低检测概率，我们不建议在没有进一步改进和测试方法的情况下使用水生或沉积eDNA进行全年采样。
The use of aquatic environmental DNA (eDNA) to detect the presence of species depends on the seasonal activity of the species in the sampled habitat. eDNA may persist in sediments for longer than it does in water, and analysing sediment could potentially extend the seasonal window for species assessment. Using the great crested newt as a model, we compare how detection probability changes across the seasons in eDNA samples collected from both pond water and pond sediments. Detection of both aquatic and sedimentary eDNA varied through the year, peaking in the summer (July), with its lowest point in the winter (January): in all seasons, detection probability of eDNA from water exceeded that from sediment. Detection probability of eDNA also varied between study areas, and according to great crested newt habitat suitability and sediment type. As aquatic and sedimentary eDNA show the same seasonal fluctuations, the patterns observed in both sample types likely reflect current or recent presence of the target species. However, given the low detection probabilities found in the autumn and winter we would not recommend using either aquatic or sedimentary eDNA for year-round sampling without further refinement and testing of the methods.