Mutation of a Cuticular Protein, BmorCPR2, Alters Larval Body Shape and Adaptability in Silkworm, Bombyx mori
Mutation of a Cuticular Protein, BmorCPR2, Alters Larval Body Shape and Adaptability in Silkworm, Bombyx mori
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角质层蛋白 BmorCPR2 的突变改变了家蚕幼虫的体型和适应性
DOI：
10.1534/genetics.113.158766
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发表时间：
2014-04-01
期刊：
影响因子：
3.3
通讯作者：
Dai, Fang-yin
中科院分区：
文献类型：
--
作者：
Qiao, Liang;Xiong, Gao;Dai, Fang-yin
角质层蛋白是昆虫表皮的重要组成部分。尽管迄今为止在已知的昆虫基因组中已经鉴定了许多编码表皮蛋白的基因，但它们在维持昆虫体形和适应性方面的功能仍然很大程度上未知。在目前的研究中，定位克隆导致识别一个基因编码的RR 1型表皮蛋白，BmorCPR 2，高度表达的幼虫几丁质丰富的组织和桑葚吃的阶段，这是负责的家蚕石质突变体。与野生型大造株相比，在大造-石株中，BmorCPR 2等位基因是具有显著较低表达的缺失突变。功能障碍BmorCPR 2在石质突变体失去几丁质结合能力，导致幼虫角质层几丁质含量减少，限制角质层延伸，减少角质层拉伸性能，节间和节间褶皱之间的比例异常。这些变化导致角质层在幼虫发育过程中容纳不断生长的内脏器官的能力显著下降，导致全身僵硬，紧密度和硬度，膨胀的节间褶皱，以及幼虫适应性的严重缺陷。据我们所知，这是第一个研究报告的相应表型的昆虫石的突变引起的RR 1型表皮蛋白。我们的研究结果共同揭示了表皮蛋白在维持正常幼虫体形方面的具体作用，并将有助于制定鳞翅目害虫防治策略。
Cuticular proteins (CPs) are crucial components of the insect cuticle. Although numerous genes encoding cuticular proteins have been identified in known insect genomes to date, their functions in maintaining insect body shape and adaptability remain largely unknown. In the current study, positional cloning led to the identification of a gene encoding an RR1-type cuticular protein, BmorCPR2, highly expressed in larval chitin-rich tissues and at the mulberry leaf-eating stages, which is responsible for the silkworm stony mutant. In the Dazao-stony strain, the BmorCPR2 allele is a deletion mutation with significantly lower expression, compared to the wild-type Dazao strain. Dysfunctional BmorCPR2 in the stony mutant lost chitin binding ability, leading to reduced chitin content in larval cuticle, limitation of cuticle extension, abatement of cuticle tensile properties, and aberrant ratio between internodes and intersegmental folds. These variations induce a significant decrease in cuticle capacity to hold the growing internal organs in the larval development process, resulting in whole-body stiffness, tightness, and hardness, bulging intersegmental folds, and serious defects in larval adaptability. To our knowledge, this is the first study to report the corresponding phenotype of stony in insects caused by mutation of RR1-type cuticular protein. Our findings collectively shed light on the specific role of cuticular proteins in maintaining normal larval body shape and will aid in the development of pest control strategies for the management of Lepidoptera.