Bacterial contamination, bacterial profile and antimicrobial susceptibility pattern of isolates from stethoscopes at Jimma University Specialized Hospital
Bacterial contamination, bacterial profile and antimicrobial susceptibility pattern of isolates from stethoscopes at Jimma University Specialized Hospital
复制标题
DOI：
10.1186/1476-0711-12-39
复制
发表时间：
2013-12-13
影响因子：
5.7
通讯作者：
Sewunet, Tsegaye
中科院分区：
文献类型：
--
作者：
Shiferaw, Teklu;Beyene, Getenet;Sewunet, Tsegaye
引言：医院获得性感染被认为是严重的公共卫生问题。感染通常是由居住在医疗环境中的生物体引起的，包括受污染的医疗设备，如Stethoscopes.Objective：确定细菌污染，细菌谱和抗生素敏感性模式的菌株从听诊器在Jimma大学Specialized Hospital.Methodology：横断面研究进行了2011年5月至9月在Jimma大学Specialized Hospital。一百七十六个听诊器拥有的卫生保健工作者（HCWs）和医学生随机选择和研究。采用自填式结构问卷收集社会人口学资料。使用湿润的无菌棉签采集标本，并使用1 ml生理盐水运输标本，所有实验室研究均在Jimma University微生物实验室按照标准微生物学技术进行。采用SPSSwindows 16版进行数据分析，P < 0.05为有统计学意义。结果：共检出污染听诊器151件，污染率为85.8%。共分离出256株细菌菌株，平均1.44x10（4）CFU/听诊器隔膜。在256株分离株中，133株（52%）为潜在病原体，如S。金黄色，克雷伯氏菌属，柠檬酸杆菌属，沙门氏菌属，变形杆菌属，肠杆菌属，铜绿假单胞菌和E.杆菌所有菌株均对多种抗生素（2至8种抗生素）耐药。消毒措施很差。消毒方式与听诊器细菌污染有关（P < 0.05）。从未消毒过的听诊器中，污染率较高的有100件（90.9%），而消毒前1周以内的听诊器中，污染率最低的有29件（72.2%）。分离株对多种抗生素耐药，是潜在的病原菌。听诊器是患者和医护人员之间传播感染的潜在媒介。听诊器隔膜在每次接触病人前后都应消毒。
Introduction: Hospital acquired infections are recognized as critical public health problems. Infections are frequently caused by organisms residing in healthcare environment, including contaminated medical equipment like Stethoscopes.Objective: To determine bacterial contamination, bacterial profile and anti-microbial susceptibility pattern of the isolates from stethoscopes at Jimma University Specialized Hospital.Methodology: Cross-sectional study conducted from May to September 2011 at Jimma University Specialized Hospital. One hundred seventy-six stethoscopes owned by Health Care Workers (HCWs) and Medical students were randomly selected and studied. Self-administered structured questionnaire was used to collect socio-demographic data. Specimen was collected using moisten sterile cotton swab and 1 ml normal saline was used to transport the specimen, all laboratory investigations were done following standard microbiological techniques, at Microbiology Laboratory, Jimma University. SPSS windows version 16 used for data analysis and P < 0.05 was considered statistically significant. Result: A total, of 151 (85.8%) stethoscopes were contaminated. A total of 256 bacterial strains and a mean of 1.44x10(4) CFUs/diaphragm of stethoscopes was isolated. Of the 256 isolates, 133 (52%) were potential pathogens like S. aureus, Klebsiella spp., Citrobacter spp., Salmonella spp., Proteus spp., Enterobacter spp., P. aeruginosa and E. coli. All strains were resistant to multiple classes of antibiotics (two to eight classes of antibiotics). Disinfection practice was poor. Disinfection practice was found to be associated with bacterial contamination of stethoscopes (P < 0.05). High contamination rate 100 (90.9%) was observed among stethoscopes that had never been disinfected; while the least contamination 29 (72.2%) was found on those disinfected a week or less before the survey.Conclusion: Bacterial contamination of the stethoscope was significant. The isolates were potential pathogens and resistant to multiple classes of antibiotics. Stethoscope is potential vehicle in the transmission of infections between patients and Healthcare Workers. Stethoscope diaphragm should be disinfected before and after each patient contact.