REACTION OF MERCURY (II) WITH NUCLEOSIDES
REACTION OF MERCURY (II) WITH NUCLEOSIDES
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DOI：
10.1021/ja00907a025
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发表时间：
1963-01-01
影响因子：
15
通讯作者：
CLARK, P
中科院分区：
文献类型：
--
作者：
EICHHORN, GL;CLARK, P
汞与核苷的附着已通过各种技术加以阐明。滴定研究表明，结合发生在尿苷和鸟苷的NiO»基团上。通过与甲醛反应掩盖氨基的实验表明，汞与腺苷和胞苷中的氨基结合。这些结果被分光光度法所证实。卡茨提出的汞- dna结构得到了汞- dna溶液滴定数据的支持。当认识到各种金属在各种条件下可以稳定二级和三级大分子结构1或使这些结构变得不稳定，2甚至导致一级结构的分解时，了解金属离子与核酸相互作用的重要性就变得明显了。3 4
The attachment of mercury to the nucleosides has been elucidated by a variety of techniques. Titration studies reveal that binding occurs at the NiO» grouping of uridine and guanosine. Experiments in which the amino group is masked by reaction with formaldehyde show that mercury is bound to the amino group in adeno-sine and cytidine. These results are confirmed by spectrophotometric observations. Support for the mercury-DNA structure proposed by Katz is obtained from titration data with mercury-DNA solutions.The importance of an understanding of the interaction of metal ions with the nucleic acids becomes evident when it is realized that metals of various kinds, under various conditions, can stabilize the secondary and tertiary macromolecular structures, 1 or labilize these structures, 2 or even bring about the decomposition of the primary structure. 3 4