Resveratrol Facilitates Pain Attenuation in a Rat Model of Neuropathic Pain Through the Activation of Spinal Sirt1
Resveratrol Facilitates Pain Attenuation in a Rat Model of Neuropathic Pain Through the Activation of Spinal Sirt1
复制标题
白藜芦醇通过激活脊髓 Sirt1 促进神经性疼痛大鼠模型的疼痛减轻
DOI：
10.1097/aap.0b013e3182795b23
复制
发表时间：
2013-03-01
影响因子：
5.1
通讯作者：
Yan, Chang-Dong
中科院分区：
文献类型：
--
作者：
Yin, Qin;Lu, Fei-Fei;Yan, Chang-Dong
关于Sirt1（一种经典的III型HDAC）在神经性疼痛中的意义的研究很少。本研究旨在探讨脊髓Sirt1和乙酰组蛋白H3在慢性收缩损伤大鼠模型中的表达变化。方法建立Sprague-Dawley大鼠单侧后肢慢性缩窄性损伤（CCI）神经性疼痛模型。结果Western blot和免疫组化分析显示，CCI术后脊髓Sirt1（沉默信息调节因子）表达降低，而乙酰组蛋白H3表达升高。鞘内给予白藜芦醇（Sirt1的激活剂），减轻cci诱导的机械异常性痛和热痛觉过敏，增加Sirt1表达，降低脊柱乙酰组蛋白H3。白藜芦醇未引起脊髓明显的组织病理学改变。结论我们的数据为脊髓Sirt1在神经性疼痛的发生和维持中所起的作用提供了新的证据。白藜芦醇的抗伤感受作用可能是通过激活CCI大鼠的脊髓Sirt1介导的。
Background Little research has been conducted regarding the implications of Sirt1 (a classic III HDAC) in neuropathic pain. The aim of this study was to investigate the variation in the expressions of spinal Sirt1 and acetyl-histone H3 in a rat model of chronic constriction injury. Methods A neuropathic pain model of chronic constriction injury (CCI) was established in a unilateral hind limb in Sprague-Dawley rats. Results Western blot analysis and immunohistochemistry revealed that Sirt1 (silent information regulator) expression decreased, whereas acetyl-histone H3 increased in the spinal cord following CCI surgery. The intrathecal administration of resveratrol, an activator of Sirt1, attenuated CCI-induced mechanical allodynia and thermal hyperalgesia, increased Sirt1 expression, and decreased acetyl-histone H3 in the spine. Resveratrol induced no obvious histopathological changes in the spinal cord. Conclusions Our data provide new evidence for the contribution of spinal Sirt1 to the initiation and maintenance of neuropathic pain. The antinociceptive effects of resveratrol may be mediated through the activation of spinal Sirt1 in CCI rats.