Ultrasensitive and selective assay of glutathione species in arsenic trioxide-treated leukemia HL-60 cell line by molecularly imprinted polymer decorated electrochemical sensors
Ultrasensitive and selective assay of glutathione species in arsenic trioxide-treated leukemia HL-60 cell line by molecularly imprinted polymer decorated electrochemical sensors
复制标题
通过分子印迹聚合物修饰的电化学传感器对三氧化二砷治疗的白血病 HL-60 细胞系中的谷胱甘肽进行超灵敏和选择性测定
DOI：
10.1016/j.bios.2016.02.017
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发表时间：
2016
影响因子：
12.6
通讯作者：
Li Yingchun
中科院分区：
文献类型：
--
作者：
Zhang Bo;Liu Jie;Ma Xiaoru;Zuo Peng;Ye Bang-ce;Li Yingchun
本文报道了一对分子印迹聚合物修饰的电化学传感器用于检测三氧化二砷处理的HL-60细胞中的谷胱甘肽（GSH）和谷胱甘肽二硫化物（GSSG）。采用电聚合的方法在电极表面原位合成了MIP膜。利用循环伏安法（CV）和电化学阻抗谱法（EIS）研究了所制备传感器的特性。GSH-MIP和GSSG-MIP传感器均具有较宽的线性检测范围和较低的检测限1.33×10−10M （S/N=3）。发现谷胱甘肽的前体n -乙酰半胱氨酸和dl -同型半胱氨酸对谷胱甘肽种类的检测影响不大，亚砷酸盐和谷胱甘肽的反应物也不受影响。该方法成功地应用于细胞样品中GSH和GSSG的鉴别，回收率为92.0 ~ 109.1%，结果与经典邻苯二醛荧光分光光度法相当。此外，所提出的传感器允许通过谷胱甘肽物种分析，白血病细胞恶性表型的逆转容易披露。
Herein a pair of molecularly imprinted polymer (MIP) modified electrochemical sensors were reported to detect glutathione (GSH) and glutathione disulfide (GSSG) in arsenic trioxide-treated HL-60 cells. MIP film was in situ synthesized onto electrode surface via electro-polymerization in a facile way. The characteristics of the obtained sensors were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Both GSH-MIP and GSSG-MIP sensors exhibit the relatively wide linear detection range and low detection limit of 1.33×10−10M (S/N=3). It is found that N-acetylcysteine and DL-homocysteine, the precursors of GSH, show little influence on the detection of glutathione species, nor did the reactants of arsenite and GSH. Such strategies were successfully applied to discriminate GSH and GSSG in cell samples with acceptable recoveries of 92.0–109.1%, and the results are comparable with classico-phthalaldehyde fluorospectrophotometry. Moreover, the presented sensors allow for easy disclosure of the reversion of malignant phenotype in leukemia cells via glutathione species analysis.