Detection of necrosis by release of lactate dehydrogenase activity.
Detection of necrosis by release of lactate dehydrogenase activity.
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DOI：
10.1007/978-1-62703-290-2_7
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发表时间：
2013
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影响因子：
--
通讯作者：
De Rosa, Maria Jose
中科院分区：
文献类型：
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作者：
Chan, Francis Ka-Ming;Moriwaki, Kenta;De Rosa, Maria Jose
细胞凋亡和坏死是在正常和疾病病理中观察到的两种主要的细胞死亡形式。虽然有许多检测细胞凋亡的方法，但用于测量坏死的方法相对较少。坏死细胞的一个关键特征是质膜的通透性。这一事件可以通过测量乳酸脱氢酶（LDH）的释放量在组织培养环境中进行量化。当与其他方法结合时，测量LDH释放是检测坏死的有用方法。在本章中，我们描述了一步一步的程序检测LDH释放从坏死细胞使用微滴板为基础的比色吸收法。
Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrosis. A key signature for necrotic cells is the permeabilization of plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is a useful method for detection of necrosis. In this chapter, we describe the step-by-step procedure for detection of LDH release from necrotic cells using a microtiter plate based colorimetric absorbance assay.