Characteristics of Pepsin-Solubilised Collagen from the Skin of Splendid Squid (Loligo formosana)
Characteristics of Pepsin-Solubilised Collagen from the Skin of Splendid Squid (Loligo formosana)
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DOI：
10.1155/2015/482354
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发表时间：
2015-01-01
影响因子：
3
通讯作者：
Kishimura, Hideki
中科院分区：
文献类型：
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作者：
Kittiphattanabawon, Phanat;Nalinanon, Sitthipong;Kishimura, Hideki
从灿烂鱿鱼（SC）的皮肤胃蛋白酶溶解的胶原蛋白分离，部分纯化的盐沉淀和透析前的特性。SC的产率为75.3%（以干重计）。结果表明，SC在232 nm处有明显的紫外吸收峰，羟脯氨酸含量较高，纯度较高。SC的总糖含量为4.70%（干重），显著高于牛皮胶原蛋白（1.45%干重）（P < 0.05）。根据SDS-PAGE和洗脱图谱，SC可能含有混合型胶原（SQ-I型和SQ-II型），其中α和β链是主要成分。SC富含甘氨酸，亚氨基酸含量高（189个残基/1000个残基）。胰凝乳蛋白酶和赖氨酰内肽酶诱导的降解在CC中更明显，与SC相比。SC的最大转变温度（Tmax）为34.1 ℃，比CC低约7 ℃。傅立叶变换红外光谱表明，SC的三螺旋结构占主导地位的碳水化合物部分的共存。因此，鱿鱼加工的副产品，鱿鱼皮可以作为胶原蛋白生产的替代来源。
Pepsin-solubilised collagen from the skin of splendid squid (SC) was isolated, partially purified by salt precipitation and dialysis prior to characterisation. The yield of SC was 75.3% (dry weight basis). SC with high purity was obtained as shown by the distinct UV absorption peak at 232 nm and high hydroxyproline content. Total sugar content of SC was 4.70% (dry weight basis), which was higher than that of collagen from calf skin (CC) (1.45% dry weight basis) (P < 0.05). Based on SDS-PAGE and elution profile, SC might contain the mixed types of collagen (type SQ-I and type SQ-II), in which alpha- and beta-chains were the major components. SC was rich in glycine and had high content of imino acids (189 residues/1000 residues). The degradation induced by chymotrypsin and lysyl endopeptidase was more pronounced in CC, compared with SC. The maximum transition temperature (T-max) of SC was 34.1 degrees C, which was about 7 degrees C lower than that of CC. Fourier transform infrared spectra revealed that the triple-helical structure of SC was predominant with the copresence of carbohydrate moieties. Therefore, the skin of splendid squid, a byproduct from squid processing, can be an alternative source for collagen production.