Tissue microarray technology for high-throughput molecular profiling of cancer
Tissue microarray technology for high-throughput molecular profiling of cancer
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DOI：
10.1093/hmg/10.7.657
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发表时间：
2001-04-01
影响因子：
3.5
通讯作者：
Sauter, G
中科院分区：
文献类型：
--
作者：
Kallioniemi, OP;Wagner, U;Sauter, G
组织微阵列 (TMA) 技术可以同时在 DNA、RNA 或蛋白质水平上快速显示数千个组织样本中的分子靶点，该技术有助于将分子发现快速转化为临床应用。通过揭示候选基因的细胞定位、流行率和临床意义，TMA 非常适合基于基因组学的诊断和药物靶点发现。与传统技术相比，TMA 具有许多优点。分子分析的速度提高了100倍以上，珍贵的组织不会被破坏，并且可以从连续的TMA切片中分析大量的分子靶点，研究档案组织标本的能力是一个重要的优势，因为此类标本通常不适用于其他高通量基因组和蛋白质组学调查，TMA的构建和分析可以自动化，进一步提高通量，TMA技术的大部分应用来自癌症研究领域，示例包括频率分析大型肿瘤材料中的分子改变、探索肿瘤进展、识别预测或预后因素以及验证新发现的基因作为诊断和治疗靶点。
Tissue microarray (TMA) technology allows rapid visualization of molecular targets in thousands of tissue specimens at a time, either at the DNA, RNA or protein level, The technique facilitates rapid translation of molecular discoveries to clinical applications. By revealing the cellular localization, prevalence and clinical significance of candidate genes, TMAs are ideally suitable for genomics-based diagnostic and drug target discovery. TMAs have a number of advantages compared with conventional techniques. The speed of molecular analyses is increased by more than 100-fold, precious tissues are not destroyed and a very large number of molecular targets can be analyzed from consecutive TMA sections, The ability to study archival tissue specimens is an important advantage as such specimens are usually not applicable in other high-throughput genomic and proteomic surveys, Construction and analysis of TMAs can be automated, increasing the throughput even further, Most of the applications of the TMA technology have come from the field of cancer research, Examples include analysis of the frequency of molecular alterations in large tumor materials, exploration of tumor progression, identification of predictive or prognostic factors and validation of newly discovered genes as diagnostic and therapeutic targets.