Expression of cytokines and inducible nitric oxide synthase in inflamed gingival tissue
Expression of cytokines and inducible nitric oxide synthase in inflamed gingival tissue
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DOI：
10.1902/jop.2001.72.5.590
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发表时间：
2001-05-01
影响因子：
4.3
通讯作者：
Okuda, K
中科院分区：
文献类型：
--
作者：
Hirose, M;Ishihara, K;Okuda, K
背景：牙周病细菌引起牙周组织炎症。据报道，牙周病变中释放的细胞因子和一氧化氮在细菌感染中发挥保护作用，并与炎症过程相关。为了阐明牙周病细菌的定植与细胞因子之间的关系，我们评估了炎性细胞因子、趋化因子、抗炎细胞因子和诱导型一氧化氮合酶 (iNOS) 的概况以及牙周炎的主要病原体牙龈卟啉单胞菌和伴放线放线杆菌的定植。方法：炎症和健康牙龈组织中细胞因子和 iNOS 的 mRNA 表达采用逆转录聚合酶链式反应（RT-PCR）方法进行测定，并分析其谱与特定细菌检测的关系。结果：牙周病灶中白细胞介素（IL）-6和iNOS mRNA的相对表达量显着高于健康个体。探诊 (BOP) 阳性部位出血时 IL-6 mRNA 表达也显着高于 BOP 阴性部位出血。在检测到放线放线菌的位点处，IL-1α和IL-8的表达增加，但IL-10表达减少。我们发现细胞因子和iNOS mRNA的表达与牙龈卟啉单胞菌感染之间没有相关性。结论：IL-6的表达可能反映牙龈组织的炎症反应，iNOS可能参与牙周炎的炎症过程。 A. actinomycetemcomitans 或 P. gingivalis 的存在可能与 IL-1 α、IL-8 和 IL-10 的不同细胞因子谱有关。
Background: Periodontopathic bacteria induce inflammation of periodontal tissues. The cytokines and nitric oxide released in periodontal lesions have been reported to play a protective role in bacterial infection and to relate to the process of inflammation. To clarify the relationship between colonization of periodontopathic bacteria and cytokines, we evaluated profiles of inflammatory cytokines, chemokine, anti-inflammatory cytokines, and inducible nitric oxide synthase (iNOS) and colonization by Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans, which are major pathogens of periodontitis.Methods: mRNA expression of cytokines and iNOS in inflamed and healthy gingival tissue was determined using reverse transcription-polymerase chain reaction (RT-PCR), and the relationship between their profiles and the detection of specific bacteria was analyzed.Results: The relative expression of interleukin (IL)-6 and iNOS mRNAs in periodontal lesions was significantly higher than those in healthy individuals. IL-6 mRNA expression was also significantly higher at bleeding on probing (BOP)-positive sites than at BOP-negative sites. The expressions of IL-1 alpha and IL-8 increased, but IL-IO expression decreased at sites where A. actinomycetemcomitans was detected. We found no correlation between the expression of cytokine and iNOS mRNA and infection by P. gingivalis.Conclusions: The expression of IL-6 may reflect inflammation in gingival tissue, and iNOS may be involved in the inflammatory process in periodontitis. The presence of A. actinomycetemcomitans or P. gingivalis might relate to the different cytokine profiles of IL-1 alpha, IL-8, and IL-10.