Distribution of dipeptidyl-peptidase IV on keratinocytes in the margin zone of a psoriatic lesion: a comparison with hyperproliferation and aberrant differentiation markers
Distribution of dipeptidyl-peptidase IV on keratinocytes in the margin zone of a psoriatic lesion: a comparison with hyperproliferation and aberrant differentiation markers
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DOI：
10.1007/s00403-008-0862-1
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发表时间：
2008-11-01
影响因子：
3
通讯作者：
van Erp, P. E. J.
中科院分区：
文献类型：
--
作者：
van Lingen, R. G.;Poll, M. K. P.;van Erp, P. E. J.
银屑病皮肤炎症过程的特点是白细胞的涌入、过度增殖和角化细胞的异常分化受细胞因子的调节。二肽基肽酶IV （DPPIV）在银屑病病变的角质形成细胞中被上调。目的是深入了解银屑病病变发展过程中DPPIV表达和酶活性以及角化细胞增殖和分化标志物的动态变化，以研究银屑病皮肤中DPPIV上调背后的机制一致性。采用免疫组化和酶组化染色的方法对银屑病病变动态边缘区DPPIV、Ki-67抗原和角蛋白16 （K16）的表达进行了研究，并对银屑病患者临床未受损伤、早期病变和慢性病变的皮肤切片与健康志愿者进行了比较。dppiv的表达、酶活性、Ki-67抗原和K16在病变中心和内缘与临床未受损伤皮肤和健康志愿者皮肤相比显著上调。在中心和内缘之间，这种上调没有显着差异。与健康志愿者的皮肤相比，临床无症状皮肤的DPPIV酶活性明显升高。我们证明DPPIV在明显银屑病病变发生前就已表达并具有酶活性。DPPIV在银屑病皮肤中的异常分布与已知的角化细胞异常生长和分化标志物不一致，这使得DPPIV（表达和酶活性）成为一个独立的标志物。
The inflammation process in psoriatic skin is characterized by influx of leukocytes, hyperproliferation and aberrant differentiation of keratinocytes regulated via cytokines. Dipeptidyl-peptidase IV (DPPIV) is known to be upregulated on keratinocytes in the psoriatic lesion. The objective was to gain insight into dynamics of DPPIV expression and enzyme activity together with keratinocyte proliferation and differentiation markers during development of a psoriatic lesion, in order to investigate coherence in mechanisms behind the upregulation of DPPIV in psoriatic skin. The expression of DPPIV, Ki-67 antigen and keratin-16 (K16) was studied in the dynamic margin zone of the psoriatic lesion, examining skin sections of the clinically uninvolved skin, the early lesion and the chronic lesion of psoriatic patients compared to healthy volunteers using immunohistochemical and enzymehistochemical staining methods. DPPIV-expression and enzyme activity, Ki-67 antigen and K16 are significantly upregulated in the centre and inner margin of the lesion compared to clinically uninvolved skin and the healthy volunteers skin. Mutually between the centre and inner margin, this upregulation did not differ significantly. The clinical symptomless skin proved to have significantly elevated DPPIV enzyme activity compared to the skin of healthy volunteers. We demonstrate that DPPIV is expressed and enzymatically active well before the development of an overt psoriatic lesion. The abnormal DPPIV distribution in psoriatic skin does not coincide with known markers of aberrant growth and differentiation of keratinocytes, which makes DPPIV (expression and enzyme activity) a marker standing on its own.