Sorting nexin 9 (SNX9) is not essential for development and auditory function in mice.
Sorting nexin 9 (SNX9) is not essential for development and auditory function in mice.
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分选 nexin 9 (SNX9) 对于小鼠的发育和听觉功能并不重要
DOI：
10.18632/oncotarget.12040
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发表时间：
2016-10-18
期刊：
影响因子：
--
通讯作者：
Xu Z
中科院分区：
文献类型：
--
作者：
Liu C;Zhai X;Du H;Cao Y;Cao H;Wang Y;Yu X;Gao J;Xu Z
分选连接蛋白是一个进化上保守的蛋白质大家族，在内吞作用、内体分选和信号传导中发挥重要作用。分选连接蛋白9（SNX9）作为分选连接蛋白家族的重要成员，参与肌动蛋白聚合、膜微管和囊泡的形成。我们先前表明，SNX9在小鼠听毛细胞中表达，并可能调节这些细胞中的肌动蛋白聚合。为了进一步研究SNX9的生理作用，我们使用同源重组方法产生了Snx9敲除小鼠。出乎意料的是，Snx9敲除小鼠具有正常的生存能力和生育能力，并且在形态学和行为上与对照小鼠无法区分。进一步研究发现，Snx9失活对听毛细胞的形态和功能没有影响，Snx9基因敲除小鼠的听阈正常。总之，我们的数据显示，Snx 9缺陷小鼠在发育和听觉功能方面没有表现出缺陷，这表明SNX 9对小鼠发育和听力并不重要。
Sorting nexins are a large family of evolutionarily conserved proteins that play fundamental roles in endocytosis, endosomal sorting and signaling. As an important member of sorting nexin family, sorting nexin 9 (SNX9) has been shown to participate in coordinating actin polymerization with membrane tubulation and vesicle formation. We previously showed that SNX9 is expressed in mouse auditory hair cells and might regulate actin polymerization in those cells. To further examine the physiological role of SNX9, we generated Snx9 knockout mice using homologous recombination method. Unexpectedly, Snx9 knockout mice have normal viability and fertility, and are morphologically and behaviorally indistinguishable from control mice. Further investigation revealed that the morphology and function of auditory hair cells are not affected by Snx9 inactivation, and Snx9 knockout mice have normal hearing threshold. In conclusion, our data revealed that Snx9-deficient mice do not show defects in development as well as auditory function, suggesting that SNX9 is not essential for mice development and hearing.