Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus
Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus
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DOI：
10.1186/1478-811x-8-29
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发表时间：
2010-10-23
影响因子：
8.4
通讯作者：
Wahab, Rohaya Megat Abdul
中科院分区：
文献类型：
--
作者：
Ariffin, Shahrul Hisham Zainal;Abidin, Intan Zarina Zainol;Wahab, Rohaya Megat Abdul
背景：本研究的目的是确定分离的悬浮小鼠外周单核血细胞是否具有分化成两种不同类型细胞的潜力，即成骨细胞和破骨细胞。结果：分化成成骨细胞伴随着Opn基因的激活、碱性磷酸酶（ALP）活性的增加和骨结节的存在， 而破骨细胞则激活Catk基因，增加抗酒石酸酸性磷酸酶（TRAP）活性，并通过吸收坑表现出吸收活性。形态学分析分别显示了von Kossa和May-Grunwald-Giemsa染色后的成骨细胞和破骨细胞的形态。结论：综上所述，悬浮单核细胞具有分化为成熟成骨细胞和破骨细胞的潜力，因此可以归类为多能干细胞。
Background: The purpose of this study is to determine whether isolated suspension mouse peripheral mononucleated blood cells have the potential to differentiate into two distinct types of cells, i.e., osteoblasts and osteoclasts.Results: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits. Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively.Conclusions: In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.