Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets
Increased expression of endothelin B receptor in static stretch exposed porcine mitral valve leaflets
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DOI：
10.1016/j.rvsc.2006.07.009
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发表时间：
2007-04-01
影响因子：
2.4
通讯作者：
Olsen, L. H.
中科院分区：
文献类型：
--
作者：
Pedersen, L. G.;Zhao, J.;Olsen, L. H.
本研究的目的是评估机械拉伸对猪二尖瓣瓣叶ET-1和ETA-和ETB-受体表达的影响，10个猪二尖瓣瓣叶节段与匹配的对照节段一起在37 ℃的缓冲液中暴露于1.5 N的静态拉伸载荷3.5 h。结果显示，与未拉伸的瓣叶相比，拉伸后的瓣叶ETB受体的转录水平明显增加，平均值为2.23（四分位数1.37和2.70）与中位数1.56（四分位数1.38和2.17，P=0.03），而ETA受体mRNA表达牵张增加了猪二尖瓣组织ETB受体的表达。这一发现有助于更好地了解粘液瘤性二尖瓣疾病的发病机制。(c)2006爱思唯尔有限公司保留所有权利。
The aim of this study was to evaluate the effect of mechanical stretch on the expression of ET-1 and ETA- and ETB-receptors in porcine mitral valve leaflets.Leaflet segments from 10 porcine mitral valves were exposed to a static stretch load of 1.5 N for 3.5 h in buffer at 37 degrees C together with matching control segments. Subsequently, the mRNA expression of ET-I, ETA-R and ETB-R was measured by real-time RT-PCR in the chordal insertion areas.The analyses showed an increased transcription of ETB-receptors in stretch-exposed leaflet segments compared to unstretched segments median 2.23 (quartiles 1.37 and 2.70) vs. median 1.56 (quartiles 1.38 and 2.17, P=0.03) whereas the mRNA expression of ETA-receptors (P=0.90) and ET-1 (P=0.51) remained unchanged.Stretch increased the expression of ETB-receptors in porcine mitral valve leaflets. The finding could lead to a better understanding of the pathogenesis of myxomatous mitral valve disease. (c) 2006 Elsevier Ltd. All rights reserved.