Microrecirculation reactor system for characterization of immobilized enzymes

Microrecirculation reactor system for characterization of immobilized enzymes
复制标题

用于表征固定化酶的微再循环反应器系统

DOI:
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发表时间:
1980
期刊:
影响因子:
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通讯作者:
H. Swaisgood
H. Swaisgood
中科院分区:
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文献类型:
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作者:
James B. Taylor;H. Swaisgood

文献摘要

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A differential microrecirculation reactor was developed for kinetic analysis of both soluble and immobilized enzymes. The reactor system was easliy fabricated with in the laboratory from readily available materials. The disposable, small reactors allowed for in situ weight determination of the enzyme beads. Routinely, only a 1 ml liquid volume of substrate was used for each kinetic assay. The reactor system was also used for determination of partition coefficients. Both uses of the reactor system required only 5–10 min for completion of a given determination.