Isolation of human adult olfactory sphere cells as a cell source of neural progenitors.
Isolation of human adult olfactory sphere cells as a cell source of neural progenitors.
复制标题
分离人类成人嗅球细胞作为神经祖细胞的细胞来源。
DOI：
10.1016/j.scr.2015.04.006
复制
发表时间：
2015
影响因子：
1.2
通讯作者：
Yoshimine T2.
中科院分区：
文献类型：
--
作者：
Ohnishi Y1;Iwatsuki K2;Ishihara M2;Shikina T3;Shinzawa K4;Moriwaki T2;Ninomiya K2;Ohkawa T2;Umegaki M2;Kishima H2;Yoshimine T2.
嗅觉干细胞是由嗅觉粘膜产生的。不同的培养条件产生嗅觉干细胞，其根据物种和发育阶段而不同，并且具有不同的祖细胞或干细胞特征。嗅球（Olfactory spheres，OS）是嗅粘膜悬浮培养产生的祖细胞或干细胞群。在这项研究中，成年人的操作系统的产生和他们的特点进行了分析。人的嗅粘膜面积超过40 mm 2，充分产生的OS。免疫细胞化学（ICC）和荧光激活细胞分选显示人OS为AN 2和A2 B5阳性。细胞类型特异性ICC的免疫荧光分析表明，Tuj 1阳性OS细胞的数量显着升高。Tuj 1阳性细胞呈典型的神经元索马体和树突状。人OS细胞也对MAP 2呈免疫阳性。相反，RIP-，O 4-和GFAP阳性细胞很少。这些RIP，O 4和GFAP阳性细胞并不像真正的少突胶质细胞和星形胶质细胞形态。在培养物中诱导少突胶质细胞分化，人OS细胞也表达神经元标记物，但少突胶质细胞或星形胶质细胞标记物均不表达。这些发现表明，人类OS细胞在我们的培养条件下自主分化为神经元，并有可能被用作其自身再生移植物的神经祖细胞的细胞来源，避免了免疫抑制和伦理争议的需要。
Olfactory stem cells are generated from olfactory mucosa. Various culture conditions generate olfactory stem cells that differ according to species and developmental stage and have different progenitor or stem cell characteristics. Olfactory spheres (OSs) are clusters of progenitor or stem cells generated from olfactory mucosa in suspension culture. In this study, adult human OSs were generated and their characteristics analyzed. Human OSs were adequately produced from olfactory mucosa with area over 40 mm2. Immunocytochemistry (ICC) and fluorescence-activated cell sorting showed that human OSs were AN2 and A2B5-positive. Immunofluorescence analysis of cell type-specific ICC indicated that the number of Tuj1-positive OS cells was significantly elevated. Tuj1-positive cells displayed typical neuronal soma and dendritic morphology. Human OS cells were also immunopositive for MAP2. By contrast, few RIP-, O4-, and GFAP-positive cells were present. These RIP, O4, and GFAP-positive cells did not resemble bona fide oligodendrocytes and astrocytes morphologically. In culture to induce differentiation of oligodendrocytes, human OS cells also expressed neuronal markers, but neither oligodendrocyte or astrocyte markers. These findings suggest that human OS cells autonomously differentiate into neurons in our culture condition and have potential to be used as a cell source of neural progenitors for their own regenerative grafts, avoiding the need for immunosuppression and ethical controversies.