Interactions of importers in long-distance transmission of wound-induced jasmonate
Interactions of importers in long-distance transmission of wound-induced jasmonate
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DOI：
10.1080/15592324.2021.1886490
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发表时间：
2021-02-12
影响因子：
2.9
通讯作者：
Liu, Pei
中科院分区：
文献类型：
--
作者：
Li, Mengya;Yu, Guanghui;Liu, Pei
从局部受损部位向远端未受损部位传递的移动伤口信号诱导茉莉酸（jasmonic acid， JA）的增加和核心JA信号的激活，为整个植物对未来挑战的广谱抗性/免疫做好准备。我们最近研究了拟南芥茉莉酸转运蛋白（JAT）家族中的两个茉莉酸入口蛋白AtJAT3和AtJAT4，它们在这种伤害诱导的系统反应/抗性（WSR）中协同调节茉莉酸在叶片间的传播。作为半分子atp结合盒转运体，AtJAT3和AtJAT4需要形成同型二聚体或/和异源二聚体才能发挥作用。通过酵母双杂交和双分子荧光互补分析，我们发现了AtJAT3-AtJAT3、AtJAT3-AtJAT4和AtJAT4-AtJAT4对的相互作用。此外，我们提出了一个模型，其中AtJAT3/AtJAT4的同源/异源二聚体介导JA的细胞-细胞运输驱动JA信号以自传播模式长距离传输，并对未来的工作进行了展望，以加强这一模型。
Mobile wound signals transmitted from local damaged to distal undamaged sites induce upsurge of jasmonic acid (JA) and activation of core JA signaling, priming the whole plant for broad-spectrum resistance/immunity against future challenges. We recently characterized two jasmonate importers AtJAT3 and AtJAT4 in Arabidopsis thaliana jasmonate transporter (JAT) family that cooperatively regulate the transmission of JA from leaf-to-leaf in this wound-induced systemic response/resistance (WSR). As half-molecule ATP-binding cassette transporters, AtJAT3 and AtJAT4 need to form homodimers or/and heterodimer to function. Here we show interactions in AtJAT3-AtJAT3, AtJAT3-AtJAT4, and AtJAT4-AtJAT4 pairs by both yeast two-hybrid and bimolecular fluorescent complementation assays. Furthermore, we propose a model in which the homo-/hetero-dimers of AtJAT3/AtJAT4 mediated cell-cell transport of JA drives long-distance transmission of JA signal in a self-propagation mode and give perspectives on future works to reinforce this model.