An outbreak of sheep pox associated with goat poxvirus in Gansu province of China
An outbreak of sheep pox associated with goat poxvirus in Gansu province of China
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中国甘肃省爆发与山羊痘病毒相关的绵羊痘疫情
DOI：
10.1016/j.vetmic.2011.11.015
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发表时间：
2012-05-04
影响因子：
3.3
通讯作者：
Zhang, Qiang
中科院分区：
文献类型：
--
作者：
Yan, Xin-Min;Chu, Yue-Feng;Zhang, Qiang
从甘肃省一次羊痘暴发中分离到3株capv病毒。采用基于P32基因的分子方法和基于RPO30基因的种特异性PCR方法对其进行分析。将P32基因的PCR产物用hif - 1酶切后，观察到两条山羊痘病毒（GTPV）特异性的条带，并利用RPO30基因为基础的PCR扩增出一个172 bp的GTPV特异性扩增子。对P32基因的序列分析表明，位于羊痘病毒P32基因163 ~ 165位的天冬氨酸编码残基缺失3个核苷酸碱基，存在6个GTPV特有的单核苷酸取代。这些病毒在遗传上与GTPV毒株更接近，并聚集在基于P32基因构建的系统发育树的GTPV分支中。结果表明分离的病毒为GTPV。这是中国首次报告与GTPV相关的羊痘暴发。(c) 2011 Elsevier B.V.版权所有
Three strains of Capripoxviruses (CaPVs) were isolated from an outbreak of sheep pox in Gansu province of China. They were analyzed by P32 gene-based molecular methods and a species-specific PCR based on the RPO30 gene. Two bands which are specific to goat poxvirus (GTPV) were observed after the PCR products of P32 gene were digested with the endonuclease of Hinf I. Moreover, an amplicon of 172 bp, which is specific to GTPV, was amplified from the viruses by using the RPO30 gene-based PCR. Sequence analysis of the P32 genes showed that three nucleotide bases for coding residue of aspartic acid which are located at 163-165 position of P32 gene of sheep poxvirus (SPPV) were absent, and six single nucleotide substitutions which are characteristic of GTPV were present. The viruses were genetically closer to GTPV strains and clustered into the GTPV branch of the phylogenetic tree constructed on the basis of the P32 gene. The results characterized the isolated viruses as GTPV. It is the first report of an outbreak of sheep pox associated with GTPV in China. (c) 2011 Elsevier B.V. All rights reserved.