Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK
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DOI：
10.1128/jb.184.9.2344-2351.2002
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发表时间：
2002-05-01
影响因子：
3.2
通讯作者：
Tanaka, T
中科院分区：
文献类型：
--
作者：
Ogura, M;Yamaguchi, H;Tanaka, T
枯草芽孢杆菌感受态转录因子ComK是建立遗传转化感受态所必需的。为了进一步研究ComK因子，我们利用DNA微阵列技术研究了ComK因子调控的基因。除了已知依赖于ComK表达的基因外，我们还发现了许多基因或操纵子，其ComK依赖性并不为人所知。在这些基因中，我们通过使用lacZ融合证实了16个基因的ComK依赖性，其中3个基因部分依赖于ComK。转化效率显着降低，在一个smf中断突变体，虽然其他ComK依赖基因的中断并没有导致转化效率显着下降。在大多数新发现的受ComK调控的基因中发现了与ComK盒相似的核苷酸序列。
The Bacillus subtilis competence transcription factor ComK is required for establishment of competence for genetic transformation. In an attempt to study the ComK factor further, we explored the genes regulated by ComK using the DNA microarray technique. In addition to the genes known to be dependent on ComK for expression, we found many genes or operons whose ComK dependence was not known preciously. Among these genes, we confirmed the ComK dependence of 16 genes by using lacZ fusions, and three genes were partially dependent on ComK. Transformation efficiency was significantly reduced in an smf disruption mutant, although disruption of the other ComK-dependent genes did not result in significant decreases in transformation efficiency. Nucleotide sequences similar to that of the ComK box were found for most of the newly discovered genes regulated by ComK.