Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle.
Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle.
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DOI：
10.1016/j.stemcr.2016.07.004
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发表时间：
2016-08-09
影响因子：
5.9
通讯作者：
Tsuchida K
中科院分区：
文献类型：
--
作者：
Uezumi A;Nakatani M;Ikemoto-Uezumi M;Yamamoto N;Morita M;Yamaguchi A;Yamada H;Kasai T;Masuda S;Narita A;Miyagoe-Suzuki Y;Takeda S;Fukada S;Nishino I;Tsuchida K
骨骼肌包含两个不同的干/祖细胞群体。一种是卫星细胞，其充当肌肉干细胞，另一种是间充质祖细胞，其有助于肌肉发病机制，如脂肪浸润和纤维化。人类这些祖细胞的详细和准确的特征仍然难以捉摸。在这里，我们进行了全面的细胞表面蛋白分析的两个祖细胞群体居住在人类骨骼肌，并确定了三个以前未被识别的标记：CD 82和CD 318的卫星细胞和CD 201的间充质祖细胞。这些标记物区分肌源性和间充质祖细胞，并使得能够有效分离两种类型的祖细胞。功能研究表明，CD 82通过抑制过度分化来确保肌源性祖细胞的扩增和保存，而CD 201信号转导有利于间充质祖细胞的脂肪形成。因此，这里鉴定的细胞表面蛋白不仅是有用的标记物，而且是功能上重要的分子，并为人类肌肉生物学和疾病提供有价值的见解。CD 82和CD 318在人肌肉卫星细胞上表达CD 201在人肌肉中的间质间充质祖细胞上表达CD 82抑制卫星细胞的过早分化CD 201信号传导促进间充质祖细胞的脂肪形成在这篇文章中，Uezumi和同事鉴定了以前未被识别的人骨骼肌源性祖细胞的标记物：CD 82和CD 318用于卫星细胞，CD 201用于间充质祖细胞。他们证明这些标记物不仅可用于细胞识别和分离，而且在调节肌肉生成和脂肪生成方面也具有重要的功能。
Skeletal muscle contains two distinct stem/progenitor populations. One is the satellite cell, which acts as a muscle stem cell, and the other is the mesenchymal progenitor, which contributes to muscle pathogeneses such as fat infiltration and fibrosis. Detailed and accurate characterization of these progenitors in humans remains elusive. Here, we performed comprehensive cell-surface protein profiling of the two progenitor populations residing in human skeletal muscle and identified three previously unrecognized markers: CD82 and CD318 for satellite cells and CD201 for mesenchymal progenitors. These markers distinguish myogenic and mesenchymal progenitors, and enable efficient isolation of the two types of progenitors. Functional study revealed that CD82 ensures expansion and preservation of myogenic progenitors by suppressing excessive differentiation, and CD201 signaling favors adipogenesis of mesenchymal progenitors. Thus, cell-surface proteins identified here are not only useful markers but also functionally important molecules, and provide valuable insight into human muscle biology and diseases. CD82 and CD318 are expressed on human muscle satellite cells CD201 is expressed on interstitial mesenchymal progenitors in human muscle CD82 suppresses premature differentiation of satellite cells CD201 signaling favors adipogenesis of mesenchymal progenitors In this article, Uezumi and colleagues identify previously unrecognized markers of human skeletal muscle-derived progenitors: CD82 and CD318 for satellite cells and CD201 for mesenchymal progenitors. They demonstrate that these markers are not only useful for cell identification and isolation but also functionally important in the regulation of myogenesis and adipogenesis.