Ectopic bone induction on and in porous hydroxyapatite combined with collagen and bone morphogenetic protein.
Ectopic bone induction on and in porous hydroxyapatite combined with collagen and bone morphogenetic protein.
复制标题
多孔羟基磷灰石与胶原和骨形态发生蛋白结合的异位骨诱导。
DOI：
10.1097/00003086-198809000-00044
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发表时间：
1988
影响因子：
4.2
通讯作者：
Geiro Ono
中科院分区：
文献类型：
--
作者：
K. Takaoka;H. Nakahara;H. Yoshikawa;K. Masuhara;T. Tsuda;Geiro Ono
将多孔羟基磷灰石(HA-P)(直径5 mm，厚1.5 mm，孔隙率80%，平均孔径200微米)植入ddy小鼠背部肌肉中，分别注入纯化的牛皮胶原蛋白(1 mg/片)和少量肉瘤来源的半纯化骨形态发生蛋白(100微克/片)。在一周内，在与宿主组织相邻的椎间盘表面不断地形成新的异位软骨组织。植入4周后，软骨被吸收，并被含有造血骨髓的正常骨取代，盘状物被包裹在新形成的骨中。仅含胶原(HA-P/胶原)或仅含BMP(100微克/片；HA-P/BMP)的HA-P椎间盘不能诱导新的软骨或骨的形成。这些结果表明，胶原作为骨形态发生蛋白的载体在体内有效地表达了骨形态发生蛋白的生物活性，并可能作为骨形态发生蛋白的载体与合成生物材料一起使用。
Porous hydroxyapatite (HA-P) discs (5 mm in diameter; 1.5 mm thick; porosity, 80%; mean pore size, 200 micron) were impregnated with purified bovine skin collagen (1 mg/disc) and a small amount of semipurified bone morphogenetic protein (BMP) of sarcoma origin (100 micrograms/disc) and implanted into dorsal muscles of ddY mice. Within one week new ectopic cartilagenous tissue was consistently formed on the surface of the discs adjacent to the host tissue. The cartilage was resorbed and replaced by normal bone containing hematopoietic bone marrow four weeks after implantation and the discs became encased in the newly formed bone. HA-P discs impregnated with only collagen (HA-P/collagen) or only BMP (100 micrograms/disc; HA-P/BMP) did not evoke formation of new cartilage or bone. These results indicate that collagen is effective as a carrier of BMP for expression of the biologic activity of the latter in vivo and that it may be of practical use as a carrier of BMP with synthetic biomaterials.