Genetic Characterization and Classification of Human and Animal Sapoviruses.
Genetic Characterization and Classification of Human and Animal Sapoviruses.
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DOI：
10.1371/journal.pone.0156373
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wang Q
中科院分区：
文献类型：
--
作者：
Oka T;Lu Z;Phan T;Delwart EL;Saif LJ;Wang Q
Sapoviruses（SaV）是一种肠道杯状病毒，已在多种哺乳动物物种中检测到，包括人类、猪、水貂、狗、海狮、黑猩猩和大鼠。他们表现出高度的多样性。SaV基因组通常编码七种非结构蛋白（NS），包括RNA聚合酶蛋白NS 7和两种结构蛋白（VP 1和VP 2）。我们根据现有的VP 1序列将人类和动物SaV分为15个基因组（G），包括本研究中新鉴定的三个基因组。本研究采用长片段RT-PCR和5' RACE方法对一个新的基因组V（GV）、一个GVII和一个GVIII猪SaV进行了全基因组测序。我们还确定了海狮GV SaV和犬GXIII SaV的5 '-和3'-末端。虽然GIX-GXII和GXV SaV的全基因组序列尚未公布，但SaV基因组的共同特征包括：1）基因组5′端为“GTG”，5′端非翻译区较短（9~14 nt）;和2）前五个氨基酸假定的NS 1的M [A/V] S [K/R] P和NS 7和VP 1之间假定的切割位点周围的五个氨基酸FEMEG在黑猩猩中是保守的，猪（GV和GVIII）、海狮、犬和人SaV的五个基因组中的两个。与此相反，这两个氨基酸基序在三个基因组的猪（GIII，GVI和GVII），蝙蝠SaV明显不同。我们的研究结果表明，几种动物SaVs与人类SaVs具有遗传相似性。然而，SaV在人类和动物之间传播的能力是不确定的。
Sapoviruses (SaVs) are enteric caliciviruses that have been detected in multiple mammalian species, including humans, pigs, mink, dogs, sea lions, chimpanzees, and rats. They show a high level of diversity. A SaV genome commonly encodes seven nonstructural proteins (NSs), including the RNA polymerase protein NS7, and two structural proteins (VP1 and VP2). We classified human and animal SaVs into 15 genogroups (G) based on available VP1 sequences, including three newly characterized genomes from this study. We sequenced the full length genomes of one new genogroup V (GV), one GVII and one GVIII porcine SaV using long range RT-PCR including newly designed forward primers located in the conserved motifs of the putative NS3, and also 5' RACE methods. We also determined the 5’- and 3’-ends of sea lion GV SaV and canine GXIII SaV. Although the complete genomic sequences of GIX-GXII, and GXV SaVs are unavailable, common features of SaV genomes include: 1) “GTG” at the 5′-end of the genome, and a short (9~14 nt) 5′-untranslated region; and 2) the first five amino acids (M [A/V] S [K/R] P) of the putative NS1 and the five amino acids (FEMEG) surrounding the putative cleavage site between NS7 and VP1 were conserved among the chimpanzee, two of five genogroups of pig (GV and GVIII), sea lion, canine, and human SaVs. In contrast, these two amino acid motifs were clearly different in three genogroups of porcine (GIII, GVI and GVII), and bat SaVs. Our results suggest that several animal SaVs have genetic similarities to human SaVs. However, the ability of SaVs to be transmitted between humans and animals is uncertain.