Mannose selection system used for cucumber transformation
Mannose selection system used for cucumber transformation
复制标题
DOI：
10.1007/s00299-006-0156-z
复制
发表时间：
2006-09-01
影响因子：
6.2
通讯作者：
Dai, Jianwu
中科院分区：
文献类型：
--
作者：
He, Zhengquan;Duan, ZhenZhen;Dai, Jianwu
选择标记系统利用编码磷酸甘露糖异构酶的pmi基因，将6-磷酸甘露糖转化为6-磷酸果糖，适用于农杆菌介导的黄瓜（Cucumis sativus L.）转化。只有转化的细胞才能利用甘露糖作为碳源。当培养基中含有 10 g/l 甘露糖和 10 g/l 蔗糖时，获得了 23% 的最高转化率。分子、遗传分析和PMI活性测定表明，再生芽含有pmi基因，并且该基因以孟德尔方式传递给后代。结果表明，甘露糖选择系统没有抗生素或除草剂选择的缺点，可用于黄瓜农杆菌介导的转化。
The selectable marker system, which utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate, was adapted for Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.). Only transformed cells were capable of utilizing mannose as a carbon source. The highest transformation frequency of 23% was obtained with 10 g/l mannose and 10 g/l sucrose in the medium. Molecular, genetic analysis, and PMI activity assay showed that the regenerated shoots contained the pmi gene and the gene was transmitted to the progeny in a Mendelian fashion. The results indicated that the mannose selection system, which is devoid of the disadvantages of antibiotic or herbicide selection, could be used for cucumber Agrobacterium-mediated transformation.