CHANGES IN DIAPHRAGM MUSCLE COLLAGEN GENE-EXPRESSION AFTER ACUTE UNILATERAL DENERVATION
CHANGES IN DIAPHRAGM MUSCLE COLLAGEN GENE-EXPRESSION AFTER ACUTE UNILATERAL DENERVATION
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DOI：
10.1152/jappl.1995.79.4.1249
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发表时间：
1995-10-01
影响因子：
3.3
通讯作者：
VAILAS, AC
中科院分区：
文献类型：
--
作者：
GOSSELIN, LE;SIECK, GC;VAILAS, AC
本研究的目的是检查急性（3天）单侧膈肌去神经（DNV）对1）α（1）（I）和α（1）（III）前胶原mRNA水平的影响; 2）胶原浓度[羟脯氨酸（HYP）]; 3）不可还原的胶原交联羟赖氨酰吡啶啉（KP）的量;和4）肌肉的被动力-长度关系。在成年雄性Fischer 344大鼠（250-300 g）的DNV中肋区和完整（INT）半膈的肌肉节段中测量α（1）（I）和α（1）（III）前胶原mRNA水平、HYP浓度和HP量。通过以恒定速度（0.6最佳长度/s）将膈肌段从最佳长度的85%延长和缩短至115%来确定DNV和INT半膈肌的体外被动力-长度关系。DNV后3天，DNV侧膈中的α 1（I）前胶原mRNA水平比INT增加了15倍以上（P < 0.05），而DNV侧膈中的α 1（III）前胶原mRNA水平比INT增加了约6倍（P < 0.05）。胶原蛋白（HYP）浓度没有组间差异，平均8.7和8.9 μ g/mg干重DNV和INT半横膈膜，分别。此外，在DNV和INT半横膈之间，成熟的不可还原胶原交联HP的量没有差异（分别为0.66和0.76摩尔HP/摩尔胶原）。DNV和INT横膈膜在延长过程中产生的被动力量没有差异。这些数据表明，急性DNV的膈肌与骨骼肌中的两个主要的纤维胶原表型的mRNA水平的增加。然而，尽管广泛的肌肉重塑，被动力量长度关系的DNV半横膈膜是不受影响的INT肌肉相比。
The purpose of the present study was to examine the effects of acute (3 days) unilateral diaphragm denervation (DNV) on 1) levels of alpha(1)(I) and alpha(1)(III) procollagen mRNA; 2) collagen concentration [hydroxyproline (HYP)]; 3) amount of the nonreducible collagen cross-link hydroxylysylpyridinoline (KP); and 4) the passive force-length relationship of the muscle. The levels of alpha(1)(I) and a(1)(III) procollagen mRNA, HYP concentration, and amount of HP were measured in muscle segments from the midcostal region of DNV and intact (INT) hemidiaphragms of adult male Fischer 344 rats (250-300 g). The in vitro passive force-length relationship of DNV and INT hemidiaphragm was determined by lengthening and shortening the diaphragm muscle segments from 85 to 115% of optimal length at a constant velocity (0.6 optimal length/s). Three days after DNV, the level of alpha(1)(I) procollagen mRNA was increased over 15-fold in the DNV hemidiaphragm compared with INT (P < 0.05), whereas the level of alpha(1)(III) procollagen mRNA was increased by approximately sixfold in the DNV hemidiaphragm compared with INT (P < 0.05). Collagen (HYP) concentration did not differ between groups, averaging 8.7 and 8.9 mu g/mg dry wt for the DNV and INT hemidiaphragms, respectively. In addition, there was no difference in the amount of the mature nonreducible collagen cross-link HP between the DNV and INT hemidiaphragms (0.66 vs. 0.76 mole HP/mole collagen, respectively). The amount of passive force developed during lengthening did not differ between DNV and INT hemidiaphragms. These data indicate that acute DNV of the hemidiaphragm is associated with an increase in the mRNA level of the two principal fibrillar collagen phenotypes in skeletal muscle. However, despite extensive muscle remodeling, the passive force-length relationship of the DNV hemidiaphragm is unaffected compared with the INT muscle.