Citrate-based fluorescent materials for low-cost chloride sensing in the diagnosis of Cystic Fibrosis.
Citrate-based fluorescent materials for low-cost chloride sensing in the diagnosis of Cystic Fibrosis.
复制标题
DOI：
10.1039/c6sc02962k
复制
发表时间：
2017-01-01
期刊：
影响因子：
8.4
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Kim JP;Xie Z;Creer M;Liu Z;Yang J
一种新型的氯离子荧光传感器是从一个基于柠檬酸盐的合成平台衍生而来的，通过动态猝灭机制，使低成本的氯离子传感能够用于囊性纤维化的临床诊断。我们首次展示了一种使用单一荧光团的选择性多卤化物传感策略。氯化物是维持体内动态平衡的一种基本电解质，生物液中的氯化物水平异常可能预示着各种疾病，如囊性纤维化。然而，目前用于氯化物检测的分析解决方案无法满足高性能和低材料或人力成本的临床需求，阻碍了将其转化为临床环境。在这里，我们提出了一类新的荧光氯化物传感器，该传感器来自一种基于柠檬酸盐的合成平台，该平台利用动态猝灭机制。基于这个低成本的平台，我们首次展示了一种选择性传感策略，该策略使用单个荧光团同时检测多个卤化物，承诺同时实现选择性和自动化，以提高性能和降低劳动力成本。我们还展示了基于柠檬酸盐的传感器作为一种新的汗液氯化物测试方法诊断囊性纤维化的临床实用性，方法是与汗液对照进行分析验证，并对患有或不患有囊性纤维化的患者的汗液进行临床验证。最后，分子模拟研究揭示了氯离子传感背后的结构机制，有助于扩大这类荧光传感器的氯气灵敏度。因此，以柠檬酸盐为基础的荧光材料可以实现低成本、自动化的多分析系统，从而实现更简单、但准确的医疗诊断，这些诊断可以很容易地转化为临床环境。更广泛地说，这样一个简单的合成平台可以实现广泛的医疗、工业和环境应用，我们的柠檬酸盐基生物可降解聚合物具有本征荧光传感功能。
A new class of fluorescence chloride sensors are derived from a facile citrate-based synthesis platform, enabling low-cost chloride sensing for clinical diagnosis of Cystic Fibrosis through dynamic quenching mechanisms. We demonstrate for the first time a selective multi-halide sensing strategy using a single fluorophore. Chloride is an essential electrolyte that maintains homeostasis within the body, where abnormal chloride levels in biological fluids may indicate various diseases such as cystic fibrosis. However, current analytical solutions for chloride detection fail to meet the clinical needs of both high performance and low material or labor costs, hindering translation into clinical settings. Here we present a new class of fluorescence chloride sensors derived from a facile citrate-based synthesis platform that utilize dynamic quenching mechanisms. Based on this low-cost platform, we demonstrate for the first time a selective sensing strategy that uses a single fluorophore to detect multiple halides simultaneously, promising both selectivity and automation to improve performance and reduce labor costs. We also demonstrate the clinical utility of citrate-based sensors as a new sweat chloride test method for the diagnosis of cystic fibrosis by performing analytical validation with sweat controls and clinical validation with sweat from individuals with or without cystic fibrosis. Lastly, molecular modeling studies reveal the structural mechanism behind chloride sensing, serving to expand this class of fluorescence sensors with improved chloride sensitivities. Thus citrate-based fluorescent materials may enable low-cost, automated multi-analysis systems for simpler, yet accurate, point-of-care diagnostics that can be readily translated into clinical settings. More broadly, a wide range of medical, industrial, and environmental applications can be achieved with such a facile synthesis platform, demonstrated in our citrate-based biodegradable polymers with intrinsic fluorescence sensing.