Eosinophil chemotaxis assay in nasal polyps by using a novel optical device EZ-TAXIScan: Role of CC-chemokine receptor 3
Eosinophil chemotaxis assay in nasal polyps by using a novel optical device EZ-TAXIScan: Role of CC-chemokine receptor 3
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DOI：
10.1016/j.alit.2016.01.001
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发表时间：
2016-07-01
影响因子：
6.8
通讯作者：
Ishikawa, Kazuo
中科院分区：
文献类型：
--
作者：
Saito, Hidekazu;Honda, Kohei;Ishikawa, Kazuo
背景：趋化因子受体CC-趋化因子受体3(CCR3)及其主要配体嗜酸性粒细胞趋化因子、RANTES和MCP-4参与嗜酸性粒细胞趋化。目前认为CCR3在鼻息肉中嗜酸性粒细胞的募集和激活中起重要作用。我们使用一种新型的实时趋化检测装置EZ-TAXIScan检测鼻息肉提取物诱导的嗜酸性粒细胞趋化作用以及CCR3拮抗剂的作用。将息肉匀浆，并测定提取液中的嗜酸性粒细胞趋化蛋白水平。采用CD16阴性选择法从人外周血中分离纯化嗜酸性粒细胞。用EZ-TAXIScan检测鼻息肉提取物在CCR3拮抗剂作用下对嗜酸性粒细胞的趋化作用。结果：鼻息肉中嗜酸性粒细胞计数与鼻息肉提取物中嗜酸性粒细胞趋化因子水平呈显著正相关。用EZ-Taxiscan观察鼻息肉提取物刺激后嗜酸性粒细胞的趋化反应。鼻息肉提取物的趋化指数与其嗜酸性粒细胞趋化因子水平呈显著正相关。鼻息肉提取物诱导的趋化作用可被CCR3拮抗剂完全抑制，但不能被抑制PGD(2)诱导的嗜酸性粒细胞趋化的趋化受体同源分子(CRTH2)所阻断。结论：CCR3通路可能通过选择性趋化嗜酸性粒细胞在鼻息肉中起重要作用。版权所有(C)2016，日本过敏学会。爱思唯尔B.V.制作和主办。
Background: The chemokine receptor, CC-chemokine receptor 3 (CCR3), and its major ligands, eotaxin, RANTES, and MCP-4, are involved in eosinophil chemotaxis. It is thought that CCR3 plays an important role in the recruitment and activation of eosinophils in nasal polyposis. We examined nasal polyp extract-induced eosinophil chemotaxis and the effect of a CCR3 antagonist using EZ-TAXIScan, a novel real-time chemotaxis assay device.Methods: Nasal polyps were obtained from chronic rhinosinusitis (CRS) patients during surgery. The polyps were homogenized and eotaxin levels in the extracts were measured. Eosinophils were purified from human peripheral blood by the CD16 negative selection method. Nasal polyp extract-induced eosinophil chemotaxis, with or without CCR3 antagonist, was assessed by EZ-TAXIScan.Results: There was a significant positive correlation between the eosinophil counts in nasal polyp and eotaxin levels in the nasal polyp extracts. Using EZ-TAXIScan, eosinophil chemotactic responses were observed following stimulation with nasal polyp extracts. There was a significant positive correlation between the chemotactic index toward the nasal polyp extracts and their eotaxin levels. Nasal polyp extract-induced chemotaxis was completely inhibited by CCR3 antagonist but not by chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2) antagonist which inhibited PGD(2)-induced eosinophil chemotaxis.Conclusions: The CCR3 pathway may play an important role in the pathogenesis of eosinophil recruitment in nasal polyps through selective eosinophil chemotaxis. Copyright (C) 2016, Japanese Society of Allergology. Production and hosting by Elsevier B.V.