Selection signatures of litter size in Dazu black goats based on a whole genome sequencing mixed pools strategy
Selection signatures of litter size in Dazu black goats based on a whole genome sequencing mixed pools strategy
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基于全基因组测序混合池策略的大足黑山羊产羔数选择特征
DOI：
10.1007/s11033-019-04904-6
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发表时间：
2019-10-01
影响因子：
2.8
通讯作者：
Huang, Yong-Fu
中科院分区：
文献类型：
--
作者：
Guang-Xin, E.;Zhao, Yong-Ju;Huang, Yong-Fu
窝产仔数被认为是估测家畜生产力的最重要指标。由于其复杂性，产仔数的分子机制尚未阐明，这限制了利用标记辅助选择创造山羊高产群体。采用混合池策略，对31只大足黑山羊进行全基因组选择性扫描分析，筛选与产羔数相关的重要基因组区域和候选基因。采用ZHp和FST混合池分析法共鉴定出96个候选基因，包括NR6A1、STK3、IGF2BP2、AR、HMGA2、NPTX 1、ANKRD 17、DPYD、CLRB、PPP3CA、PLCB 1、STK3和HMGA2。我们根据信号通路的功能分类和注释对这些候选基因进行了分类。根据GO和KEGG分析结果，共从这些基因中注释了43个GO术语和108条通路。特别是在雌激素信号通路和卵母细胞减数分裂等生殖相关通路中发现了一些新的候选基因。这些结果为研究编码基因对山羊繁殖力性状的影响提供了新的思路。
Litter size is considered to be the most important index for estimating domestic animal productivity. Due to its complexity, the molecular mechanism of litter size has not been elucidated, and it has restricted the use of marker-assisted selection to create high-yield populations in goats. A genome-wide selective sweep analysis was performed with 31 Dazu black goats to identify significant genomic regions and candidate genes related to litter size by a mixed pools strategy. A total of 96 candidate genes were identified, including NR6A1, STK3, IGF2BP2, AR, HMGA2, NPTX1, ANKRD17, DPYD, CLRB, PPP3CA, PLCB1, STK3 and HMGA2, using mixed pool analysis with ZHp and FST. We classified these candidate genes based on the functional classification and annotation of signaling pathways. According to the GO and KEGG analysis results, a total of 43 GO terms and 108 pathways were annotated from these genes. In particular, some novel candidate genes were enriched in reproduction-related pathways, such as the estrogen signaling pathway and oocyte meiosis. These findings provide insight into the influences of coding genes on the fecundity traits of goats.