A combination of Citrus reticulata peel and black tea inhibits migration and invasion of liver cancer via PI3K/AKT and MMPs signaling pathway
A combination of Citrus reticulata peel and black tea inhibits migration and invasion of liver cancer via PI3K/AKT and MMPs signaling pathway
复制标题
陈皮与红茶组合通过PI3K/AKT和MMPs信号通路抑制肝癌迁移和侵袭
DOI：
10.1007/s11033-019-05157-z
复制
发表时间：
2019-10-30
影响因子：
2.8
通讯作者：
Sun, Shili
中科院分区：
文献类型：
--
作者：
Wen, Shuai;Sun, Lingli;Sun, Shili
肝癌是世界上最常见的恶性肿瘤之一，是导致癌症死亡的第二大原因。柑橘网状皮和红茶已被研究其有益的健康效果。尽管已经报道了许多研究，但其健康益处的潜在分子机制仍不完全清楚。本研究将陈皮与红茶混合制成陈皮红茶（CRPBT），并对其有效成分、抗氧化和抗肝癌作用进行体外评价。结果表明，CRPBT具有一定的抗氧化能力，能有效抑制肝癌细胞的增殖和迁移，并呈剂量和时间依赖性。CRPBT能显著下调肝癌细胞PI 3 K和AKT的磷酸化水平，上调Bax/Bcl-2的比值，抑制MMP 2/9、N-cadherin和Vimetin蛋白的表达。CRPBT具有良好的抑制肝癌细胞迁移、侵袭、增殖和诱导凋亡的作用。
Liver cancer, one of the most common malignancies, is the second leading cause of cancer death in the world. The citrus reticulate peel and black tea have been studied for their beneficial health effects. In spite of the many studies have been reported, the underlying molecular mechanisms underlying its health benefits are still not fully understood. In present study, we developed a unique citrus reticulate peel black tea (CRPBT) by combined citrus reticulate peel and black tea and assessed its active ingredients, anti-oxidant and anti-liver cancer effects in vitro. The results suggested that CRPBT exhibited antioxidant capacity and effectively inhibited proliferation and migration of liver cancer cells in a dose- and time- dependent manner. Mechanistically, CRPBT significantly down-regulated phosphorylation of PI3K and AKT, and up-regulated the ratio of Bax/Bcl-2, and suppressed the expression of MMP2/9, N-cadherin and Vimetin proteins in liver cancer cells. Taken together, CRPBT has good effect on inhibiting migration, invasion, proliferation, and inducing apoptosis in liver cancer cells.