The main anthocyanin monomer of Lycium ruthenicum Murray induces apoptosis through the ROS/PTEN/PI3K/Akt/caspase 3 signaling pathway in prostate cancer DU-145 cells
The main anthocyanin monomer of Lycium ruthenicum Murray induces apoptosis through the ROS/PTEN/PI3K/Akt/caspase 3 signaling pathway in prostate cancer DU-145 cells
复制标题
黑果枸杞主要花色苷单体通过ROS/PTEN/PI3K/Akt/caspase 3信号通路诱导前列腺癌DU-145细胞凋亡
DOI：
10.1039/d0fo02382e
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发表时间：
2021-02-21
期刊：
影响因子：
6.1
通讯作者：
Ran, Lin-Wu
中科院分区：
文献类型：
--
作者：
Li, Zhan-Long;Mi, Jia;Ran, Lin-Wu
花青素具有有效的化学预防活性。枸杞含有丰富的花色苷，具有多种生物活性。本研究旨在探讨枸杞花色苷单体（Pt 3G）对前列腺癌DU-145细胞的作用及其可能的生物学机制。甲基噻唑四氮唑法检测细胞增殖;采用流式细胞术和原位末端标记法检测细胞凋亡率。Western blotting检测细胞凋亡相关蛋白的表达。我们的数据表明，Pt 3G抑制细胞增殖，诱导凋亡，并促进细胞周期停滞在S期的浓度依赖性的方式（0，100，200和400 μ g mL（-1））。Pt 3G通过调节Bax和Bcl-2的表达，降低线粒体膜通透性。Western blot分析表明Pt 3G可显著增加PTEN的表达，并激活PI 3 K/Akt介导的caspase 3通路。此外，我们的研究结果还表明Pt 3G通过激活PTEN基因，刺激ROS的过度产生，从而诱导DU-145细胞凋亡。综上所述，这些结果表明Pt 3G通过ROS/PTEN/PI 3 K/Akt/caspase 3信号通路抑制前列腺癌DU-145细胞的增殖并诱导细胞凋亡。因此，枸杞Pt 3G可能是一种潜在的抗增殖剂，用于预防或治疗前列腺癌。
Anthocyanins have been reported to have effective chemopreventive activity. Lycium ruthenicum Murray is rich in anthocyanins and exhibits many biological activities. The purpose of this study was to investigate the effects and possible biological mechanism of the main anthocyanin monomer (Pt3G) of Lycium ruthenicum Murray on prostate cancer DU-145 cells. The cell proliferation was detected by methyl thiazolyl tetrazolium assay. The cell apoptosis rates were assessed by flow cytometric analysis and TUNEL assay. The expressions of apoptosis related proteins were evaluated by western blotting. Our data demonstrated that Pt3G inhibited cell proliferation, induced apoptosis and promoted cell cycle arrest at the S phase in a concentration-dependent manner (0, 100, 200 and 400 mu g mL(-1)). Furthermore, it was shown that Pt3G decreased the mitochondrial membrane permeability through regulating the expressions of Bax and Bcl-2. Western blot analysis indicated that Pt3G significantly increased the expression of PTEN and then activated the PI3K/Akt-mediated caspase 3 pathway. In addition, our results also suggested that Pt3G activated the PTEN gene to induce the apoptosis of DU-145 cells by stimulating the overproduction of ROS. To sum up, these results indicate that Pt3G inhibits cell proliferation and induces apoptosis through the ROS/PTEN/PI3K/Akt/caspase 3 signaling pathway in prostate cancer DU-145 cells. Therefore, Pt3G of Lycium ruthenicum Murray may be a potential anti-proliferative agent for the prevention or treatment of prostate cancer.