Molecular and phenotypic evidence for the spread of three major methicillin-resistant Staphylococcus aureus clones associated with two characteristic antimicrobial resistance profiles in China
Molecular and phenotypic evidence for the spread of three major methicillin-resistant Staphylococcus aureus clones associated with two characteristic antimicrobial resistance profiles in China
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三种主要耐甲氧西林金黄色葡萄球菌克隆在中国传播的分子和表型证据与两种特征性抗菌药物耐药性相关
DOI：
10.1093/jac/dkt213
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发表时间：
2013-11-01
影响因子：
5.2
通讯作者：
Rao, Xiancai
中科院分区：
文献类型：
--
作者：
Cheng, Hang;Yuan, Wenchang;Rao, Xiancai
目的：耐甲氧西林金黄色葡萄球菌（MRSA）克隆的分布是动态的和地理上独特的。为了了解中国MRSA感染的流行病学变化，我们进行了一项前瞻性的多城市监测研究，采用分子分型和表型分析，以确定主要流行克隆与其抗菌药物耐药谱的相关性。方法：对2009年1月至2012年3月从我国6个城市分离的金黄色葡萄球菌（MRSA）菌株进行PCR分析和分子分型，包括葡萄球菌盒式染色体mec分型、多位点序列分型、葡萄球菌蛋白A基因分型和PFGE分型。发现三个主要克隆在中国流行：ST 239-MRSA-III-t030、ST 239-MRSA-III-t037和ST 5-MRSA-II-t002。这三个克隆与两个特征性耐药谱相关，即第一个克隆为庆大霉素/环丙沙星/利福平/左氧氟沙星，后两个克隆为庆大霉素/环丙沙星/克林霉素/红霉素/四环素/左氧氟沙星/甲氧苄啶/磺胺甲恶唑。几个地理上独特的次要克隆也identified.Conclusions：在中国的主要MRSA克隆与特征性的抗菌药物耐药谱。可以基于菌株分型数据选择用于治疗MRSA感染患者的抗生素。
Objectives: The distribution of methicillin-resistant Staphylococcus aureus (MRSA) clones is dynamic and geographically unique. To understand the changing epidemiology of MRSA infections in China, we performed a prospective, multicity surveillance study with molecular typing and phenotypic analysis to determine the association of major prevalent clones with their antimicrobial resistance profiles.Methods: A total of 517 S. aureus isolates collected between January 2009 and March 2012 from six cities in China were subjected to antibiogram analysis and molecular typing, including staphylococcal cassette chromosome mec typing, multilocus sequence typing, staphylococcal protein A gene typing and PFGE typing.Results: Among the isolates collected, 309 were characterized as MRSA, with a prevalence of 59.8%. Three major clones were found to be prevalent in China: ST239-MRSA-III-t030, ST239-MRSA-III-t037 and ST5-MRSA-II-t002. These three clones were associated with two characteristic resistance profiles, namely, gentamicin/ciprofloxacin/rifampicin/levofloxacin for the first clone and gentamicin/ciprofloxacin/clindamycin/erythromycin/tetracycline/levofloxacin/trimethoprim/sulfamethoxazole for the latter two. Several geographically unique minor clones were also identified.Conclusions: The predominant MRSA clones in China were associated with characteristic antimicrobial resistance profiles. Antibiotics for treating patients with MRSA infections can be selected based on the strain typing data.