Immunohistochemical expression of keratan sulfate: a possible diagnostic marker for carcinomas of the female genital tract
Immunohistochemical expression of keratan sulfate: a possible diagnostic marker for carcinomas of the female genital tract
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DOI：
10.1136/jclinpath-2011-200231
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发表时间：
2011-12-01
影响因子：
3.4
通讯作者：
Shiozawa, Tanri
中科院分区：
文献类型：
--
作者：
Miyamoto, Tsutomu;Ishii, Keiko;Shiozawa, Tanri
目的：作者曾报道硫酸角质素（KS），一种糖胺聚糖，在正常和肿瘤性尿道上皮中的表达。本研究的目的是评估其作为诊断标志物的潜在用途，并研究KS在其他人类上皮组织中的表达。方法采用化学方法检测102例正常上皮和110例女性生殖道癌组织中KS的表达（FGT;子宫颈、子宫内膜、卵巢、输卵管）、消化器官结果在正常组织中，KS在FGT和异位子宫内膜中一致性表达（25/26），在消化道（1/42）和泌尿道（0/6）中未检测到，在肺（7/10）、乳腺（3/9）和甲状腺（4/4）中仅部分表达。在恶性肿瘤组织中，KS在子宫内膜癌、卵巢癌和输卵管癌中均呈阳性表达（29/32），在肺癌、乳腺癌、甲状腺癌、胰腺癌和间皮瘤中部分表达，而在胃肠道癌（0/17）、肝癌（0/5）和泌尿道癌（0/11）中未见表达。在FGT、消化道和泌尿道癌中，KS阳性提示FGT癌的可能性，其敏感性为79.5%（31/39），特异性为92.9%（39/42）。
Aims The authors previously reported the expression of keratan sulfate (KS), a glycosaminoglycan, in the epithelium of normal and neoplastic endometria. The aim of this study was to evaluate its potential use as a diagnostic marker, and the expression of KS was investigated in other human epithelial tissues.Methods Expression was examined immunohistochemically using 102 samples of normal epithelia and 110 samples of carcinomas from the female genital tract (FGT; cervix, endometrium, ovary, fallopian tube), digestive organs (gastrointestinal tract, pancreas, liver), urinary tract, lung, mammary gland, thyroid and mesothelium.Results In normal tissues, KS was consistently detected in the FGT and ectopic endometrium (25/26), but was not found in the digestive organs (1/42) and urinary tract (0/6), and was only partly detected in the lung (7/10), mammary gland (3/9) and thyroid (4/4). In malignant tissues, KS was consistently observed in carcinomas of the endometrium, ovary and fallopian tube (29/32), and was partly detected in carcinomas of the lung, mammary gland, thyroid, pancreas and mesothelium, but was absent in carcinomas of the gastrointestinal tract (0/17), liver (0/5) and urinary tract (0/11). Among carcinomas of the FGT, digestive organs and urinary tract, KS positivity suggested the possibility of FGT carcinomas, with 79.5% (31/39) sensitivity and 92.9% (39/42) specificity.Conclusions KS is a potentially useful marker for the supportive diagnosis of the primary site of metastatic carcinomas or unknown primary carcinomas, especially in the abdominal cavity.