Membranous osteogenesis system modeled with KUSA-A1 mature osteoblasts
Membranous osteogenesis system modeled with KUSA-A1 mature osteoblasts
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DOI：
10.1016/j.bbagen.2005.05.027
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发表时间：
2005-08-30
影响因子：
3
通讯作者：
Umezawa, A
中科院分区：
文献类型：
--
作者：
Matsumoto, S;Shibuya, I;Umezawa, A
从小鼠骨髓培养物中建立了几个基质细胞。KUSA亚克隆之一KUSA- al细胞在体外和体内均表现出成骨特性。KUSA-Al细胞的钙沉积、骨钙素释放和甲状旁腺激素（PTH）反应表明它们是成熟的成骨细胞或骨细胞。免疫缺陷小鼠皮下注射细胞1周后，皮下组织骨形成。kasa - al的成骨不通过软骨形成介导，因此被认为是膜性骨化。kasa - al细胞的这些独特特征为详细分析膜性骨化过程提供了机会。(c) 2005 Elsevier B.V.版权所有
Several stromal cells were established from murine bone marrow cultures. One of the KUSA subclones, KUSA-Al cells, displays osteogenic characteristics in vitro and in vivo. The calcium deposition, osteocalcin release, and parathyroid hormone (PTH) responsiveness of KUSA-Al cells indicate that they are mature osteoblasts or osteocytes. Bone had formed in subcutaneous tissue 1 week after subcutaneous injection of cells into inummodeficient mice. The osteogenesis by KUSA-Al was not mediated by chondrogenesis and thus was considered to be membranous ossification. These unique characteristics of KUSA-Al cells provide an opportunity to analyze the process of membranous ossification in detail. (c) 2005 Elsevier B.V. All rights reserved.