The synergistic effects of waterborne microcystin-LR and nitrite on hepatic pathological damage, lipid peroxidation and antioxidant responses of male zebrafish
The synergistic effects of waterborne microcystin-LR and nitrite on hepatic pathological damage, lipid peroxidation and antioxidant responses of male zebrafish
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水性微囊藻毒素-LR和亚硝酸盐对雄性斑马鱼肝脏病理损伤、脂质过氧化和抗氧化反应的协同作用
DOI：
10.1016/j.envpol.2017.12.059
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发表时间：
2018-04-01
影响因子：
8.9
通讯作者：
Tang, Rong
中科院分区：
文献类型：
--
作者：
Lin, Wang;Hou, Jie;Tang, Rong
腐烂的蓝细菌开花中的危险物质，例如微囊蛋白藻（MC-LR）和亚硝酸盐对水生生物构成严重的挑战。为了评估MC-LR和亚硝酸盐对肝病理学，脂质过氧化和鱼类的抗氧化反应的联合毒性作用，成年雄性斑马鱼（Danio Rerio）暴露于具有不同浓度的MC-LR的溶液中（0，3，30 Mu G /l）和亚硝酸盐（0，2，20 mg/l）30 d。结果表明，随着单个因子MC-LR或亚硝酸盐浓度的增加，肝病理病变在严重程度和程度上进展，并在共曝光组中变得更加严重。同时，丙二醛（MDA）的显着增加表明，由MC-LR，亚硝酸盐及其两者引起的氧化应激发生，这是通过总抗氧化能力（T-AOC）以及转录和转录和转录和转录和转录和转录和转录和转录和转录和转录能力的显着降低来验证的。抗氧化剂酶（CAT和GPX）的活性。肝线粒体被破坏为MC-LR和亚硝酸盐的共同作用位点，这表明氧化应激在MC-LR和亚硝酸盐的肝毒性机制中起着重要作用。肝谷胱甘肽（GSH）的耗竭表明GSH/谷胱甘肽-S-转移酶（GST）系统在这两种化学物质排毒中的重要性。这些结果清楚地表明，MC-LR和亚硝酸盐对斑马鱼肝脏中的组结构，抗氧化能力和排毒能力具有协同作用。因此，富营养湖泊中MC-LR和亚硝酸盐的污染综合可以减少鱼类的防御机制并加速GSH的消耗，GSH的消耗损害了长时间的蓝细菌花朵发作期间鱼的存活率。 （c）2017 Elsevier Ltd.保留所有权利。
Hazardous materials from decaying cyanobacterial blooms, such as microcystin-LR (MC-LR) and nitrite pose serious challenges to aquatic organisms. To assess combined toxic effects of MC-LR and nitrite on hepatic pathology, lipid peroxidation and antioxidant responses of fish, adult male zebrafish (Danio rerio) were exposed to solutions with different combined concentrations of MC-LR (0, 3, 30 mu g/L) and nitrite (0, 2, 20 mg/L) for 30 d. The results showed that hepatic pathological lesions progressed in severity and extent with increasing concentration of single factor MC-LR or nitrite and became more severe in co-exposure groups. Concurrently, significant increases in malondialdehyde (MDA) revealed the occurrence of oxidative stress caused by MC-LR, nitrite and both of them, which was indirectly verified by remarkable decreases in the total antioxidant capacity (T-AOC) as well as the transcription and activity of antioxidant enzymes (CAT and GPx). Hepatic mitochondria were damaged as the common action site of MC-LR and nitrite, suggesting that oxidative stress played a significant role in the mechanisms of the hepatotoxicity of MC-LR and nitrite. The depletion of hepatic glutathione (GSH) indicated the importance of GSH/glutathione-S-transferases (GST) system in these two chemicals detoxification. These results clearly illustrated that MC-LR and nitrite have synergistic effects on the histostructure, antioxidant capacity and detoxification capability in the liver of zebrafish. Therefore, the combined pollution of MC-LR and nitrite in eutrophic lakes can reduce the defense mechanism of the fish and accelerate the consumption of GSH, which compromise the survival of the fish during prolonged cyanobacterial blooms episodes. (C) 2017 Elsevier Ltd. All rights reserved.