Induction of Nesidioblastosis Will Reverse Diabetes in Syrian Golden Hamster
Induction of Nesidioblastosis Will Reverse Diabetes in Syrian Golden Hamster
复制标题
诱导成纤维细胞增多症将逆转叙利亚金仓鼠的糖尿病
作者：
L. Rosenberg;William P. Duguid;Rea A. Brown;A. Vinik
胰岛细胞增多症是新胰岛的形成和胰岛内细胞的分化，代表临床水平上朗格汉斯胰岛功能亢进状态的一部分。叙利亚金仓鼠的胰岛细胞增多症可以通过用玻璃纸胶带包裹胰头来诱发。不涉及导管结扎，并且不发生腺泡细胞萎缩。本研究的目的是确定诱导成纤维细胞增多症是否可以用作逆转链佐星诱导的糖尿病的一种手段。 8 周龄的远交仓鼠 (n = 32) 通过腹腔注射 40 mg/kg 治疗而患上糖尿病。链脲佐菌素，每天给药，持续 3 天。四天后，随机选择 16 只动物进行剖腹手术，并用玻璃纸包裹胰腺。手术前，未手术对照动物（389.0±18.6、33.9±3.8）的血清葡萄糖和胰岛素水平（平均值±SE）与等待手术的动物（373.2±18.6、37.9±3.8）没有差异。 7 周后，50% 的手术动物的血清葡萄糖和胰岛素水平正常，而未手术的对照动物中只有 12% (χ2 = 5.53，P < .05)。来自正常血糖手术动物的胰岛的特征是数量增加，包括许多小胰岛、免疫反应性胰岛素染色呈阳性和细胞空泡化程度最小。来自高血糖手术仓鼠和未手术对照动物的胰岛数量减少，尺寸通常较大，表现出很少或没有免疫反应性胰岛素染色，并且表现出明显的细胞空泡化。结论是，胰腺的玻璃纸包裹诱导了胰岛和内分泌细胞的形成，这些细胞在功能上能够逆转链佐星诱导的糖尿病。
Nesidioblastosis, which is the formation of new islets and the differentiation of cells within the islets, represents part of the spectrum of hyperfunctioning states of the islets of Langerhans at the clinical level. Nesidioblastosis in the Syrian golden hamster can be induced by wrapping the head of the pancreas with cellophane tape. Ligation of the duct is not involved, and acinar cell atrophy does not occur. The purpose of this study was to determine whether the induction of nesidioblastosis could be used as a means of reversing streptozocin-induced diabetes. Outbred hamsters (n = 32), 8 wk of age, were rendered diabetic by treatment with 40 mg/kg i.p. streptozocin, administered daily for 3 days. Four days later, 16 animals chosen at random underwent laparotomy with cellophane wrapping of the pancreas. Before surgery, the serum glucose and insulin levels (means ± SE) in the unoperated control animals (389.0 ± 18.6, 33.9 ± 3.8) did not differ from those in the animals awaiting the operation (373.2 ± 18.6, 37.9 ± 3.8). After 7 wk, 50% of the operated animals had serum glucose and insulin levels that were normal, compared to only 12% of the unoperated control animals (χ2 = 5.53, P < .05). Islets from normoglycemic operated animals were characterized by increased numbers, including many small islets, positive immunoreactive insulin staining, and minimal vacuolation of cells. Islets from hyperglycemic operated hamsters and from the unoperated control animals were decreased in number and generally larger in size, demonstrated little or no immunoreactive insulin staining, and exhibited marked vacuolation of cells. It is concluded that cellophane wrapping of the pancreas induces the formation of islets and endocrine cells that are functionally capable of reversing streptozocin-induced diabetes.