The flavonoid quercetin inhibits pancreatic cancer growth in vitro and in vivo.
The flavonoid quercetin inhibits pancreatic cancer growth in vitro and in vivo.
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DOI：
10.1097/mpa.0b013e318264ccae
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发表时间：
2013-03
期刊：
影响因子：
2.9
通讯作者：
Hines OJ
中科院分区：
文献类型：
--
作者：
Angst E;Park JL;Moro A;Lu QY;Lu X;Li G;King J;Chen M;Reber HA;Go VL;Eibl G;Hines OJ
在几个临床前动物模型中，黄酮类QUCETIN有望成为抗肿瘤药物。然而，口服槲皮素对胰腺癌小鼠模型的疗效尚不清楚。采用细胞计数和四甲基偶氮唑盐比色法检测了槲皮素单用或与吉西他滨合用对两种人胰腺癌细胞株的抗增殖作用。流式细胞仪检测细胞凋亡率。用生物发光法研究了原位胰腺癌动物模型体内肿瘤的生长情况。在饮食中口服槲皮素。槲皮素能抑制胰腺癌细胞系的生长，这是通过诱导细胞凋亡而引起的。此外，饮食中添加槲皮素可抑制原位移植胰腺移植物的生长。吉西他滨和槲皮素联合使用与单独使用槲皮素相比没有额外的效果。在体内，Quercetin可引起明显的细胞凋亡，并抑制肿瘤细胞的增殖。我们的数据提供了口服给药能够抑制裸鼠原位胰腺肿瘤生长的证据。这些数据表明，槲皮素可能对胰腺癌患者有好处。
The flavonoid quercetin holds promise as an anti-tumor agent in several preclinical animal models. However, the efficacy of oral administration of quercetin in a pancreatic cancer mouse model is unknown. The anti-proliferative effects of quercetin alone or in combination with gemcitabine were tested in two human pancreatic cancer cell lines using cell count and MTT assays. Apoptosis was evaluated by flow cytometry. Tumor growth in vivo was investigated in an orthotopic pancreatic cancer animal model using bioluminescence. Quercetin was administered orally in the diet. Quercetin inhibited the growth of pancreatic cancer cell lines, which was caused by an induction of apoptosis. In addition, dietary supplementation of quercetin attenuated the growth of orthotopically transplanted pancreatic xenografts. The combination of gemcitabine and quercetin had no additional effect compared to quercetin alone. In vivo quercetin caused significant apoptosis and reduced tumor cell proliferation. Our data provide evidence that oral administration of quercetin was capable of inhibiting growth of orthotopic pancreatic tumors in a nude mouse model. These data suggest a possible benefit of quercetin in patients with pancreatic cancer.