Simple analysis of blonanserin, a novel antipsychotic agent, in human plasma by GC-MS
Simple analysis of blonanserin, a novel antipsychotic agent, in human plasma by GC-MS
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DOI：
10.1007/s11419-010-0090-1
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发表时间：
2010-07-01
影响因子：
2.2
通讯作者：
Seno, Hiroshi
中科院分区：
文献类型：
--
作者：
Hattori, Hideki;Iwai, Masae;Seno, Hiroshi
布南色林是一种具有多巴胺D-2和5-羟色胺5-HT 2A受体拮抗剂性质的新型抗精神病药。本文介绍了一种以己巴比妥为内标物，用气相色谱-质谱联用（GC-MS）分析血浆中布南色林的方法。将含有布南色林和50 ng IS的1 ml血浆与0.5 ml 0.1 M盐酸和2.5 ml蒸馏水充分混合;将混合物加到Oasis HLB柱中。用5 ml蒸馏水洗涤柱，用2 ml氯仿洗脱目标化合物和IS。在氮气流下将混合物蒸发至干。将残留物在50 μ l甲醇中复溶，用于GC-MS分析。加标至人血浆中的布南色林的校准曲线在0.5-20 ng/ml范围内显示出良好的线性。检测限（信噪比= 3）约为0.25 ng/ml。2.5 ng/ml的回收率为81.0%，15 ng/ml的回收率为97.5%。准确度和精密度也令人满意。一名60岁的男性志愿者口服10 mg布南色林，在摄入后1.5和4 h，该药物的血浆浓度分别为0.61和1.20 ng/ml。
Blonanserin is a novel antipsychotic agent having dopamine D-2 and serotonin 5-HT2A receptor antagonist properties. In this communication, a simple method for analysis of blonanserin in human plasma by gas chromatography-mass spectrometry (GC-MS) using hexobarbital as internal standard (IS) is presented. One milliliter of plasma containing blonanserin and 50 ng of IS was mixed well with 0.5 ml of 0.1 M hydrochloric acid and 2.5 ml of distilled water; the mixture was applied to an Oasis HLB cartridge. The cartridge was washed with 5 ml of distilled water, and the target compound and IS were eluted with 2 ml of chloroform. The eluate was evaporated to dryness under a stream of nitrogen. The residue was reconstituted in 50 mu l of methanol for use in GC-MS analysis. The calibration curve for blonanserin spiked into human plasma showed good linearity in the range of 0.5-20 ng/ml. The detection limit (signal-to-noise ratio = 3) was about 0.25 ng/ml. The recovery rate was 81.0% at 2.5 ng/ml and 97.5% at 15 ng/ml. The accuracy and precision were also satisfactory. A 60-year-old male volunteer took a 10-mg oral dose of blonanserin; the plasma concentrations of the drug were 0.61 and 1.20 ng/ml at 1.5 and 4 h after ingestion, respectively.