Rapid tests to assess the antioxidant activity of Phaseolus vulgaris L. dry beans
Rapid tests to assess the antioxidant activity of Phaseolus vulgaris L. dry beans
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DOI：
10.1021/jf049001r
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发表时间：
2005-04-20
影响因子：
6.1
通讯作者：
Romani, A
中科院分区：
文献类型：
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作者：
Heimler, D;Vignolini, P;Romani, A
对干豆的抗自由基活性进行了评估，以评估该测试的有效性，并将测试结果与Flin-Ciocalteu法和快速分光光度法测定总黄酮的结果相关联。对意大利托斯卡纳和巴西卡塔两个地区3年间采集的4个菜豆地方品种(12个样本)进行了分析。EC50值范围为39~2810 mg样品/mg 1，1-二苯基-2-苦基肼自由基。用没食子酸当量表示各样品的酚类物质含量，其变化范围为1.17~4.40 mg/g；类黄酮含量以(+)-儿茶素的mg/g表示，变化范围为0.24~1.43 mg/g。多酚类物质的定性组成也已通过高效液相色谱-二极管阵列检测和LC/MS确定。这些研究表明，快速检测有助于评价保健食品的质量。
The antiradical activity of dry beans was evaluated in order to assess the validity of this test and to correlate the results with those achieved with the Folin-Ciocalteu method and with a rapid spectrophotometric method for the analysis of total flavonoids. Four landraces (12 samples) of common beans (Phaseolus vulgaris L.), collected in two regions of Italy (Tuscany and Basilicata) in three different years, were analyzed. The EC50 values ranged from 39 to 2810 mg sample/mg 1,1-diphenyl-2-picrylhydrazil radical. The phenolic content of each sample was expressed as gallic acid equivalents; it changed from 1.17 to 4.40 mg/g. The flavonoid content, expressed as mg of (+)-catechin per g of dry seeds, ranged from 0.24 to 1.43 mg/g. The qualiquantitative composition of polyphenols has been also elucidated by means of high-performance liquid chromatography (HPLC)-diode array detection and HPLC/MS. These investigations showed that rapid tests can contribute to assessing the quality of functional food.