Spontaneous oscillatory burst activity in the piriform–amygdala region and its relation to in vitro respiratory activity in newborn rats
Spontaneous oscillatory burst activity in the piriform–amygdala region and its relation to in vitro respiratory activity in newborn rats
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DOI：
10.1016/j.neuroscience.2006.09.033
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发表时间：
2007-01
期刊：
影响因子：
3.3
通讯作者：
H. Onimaru;I. Homma
中科院分区：
文献类型：
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作者：
H. Onimaru;I. Homma
杏仁核对于受嗅觉信息影响的情绪的形成非常重要。梨状皮质参与与嗅觉有关的信息处理。为了研究梨状皮质和杏仁核之间的功能相互作用及其与延髓呼吸活动的关系，我们开发了一种新的体外制备方法，包括新生大鼠的边缘系统、脑干和脊髓。使用光学和电生理记录，我们分析了自发性神经元活动的梨状核杏仁核复合体在边缘脑干脊髓制备0至1天的大鼠。对于光学记录，用电压敏感染料对制备物进行染色，并从第四颈（C4）前根监测吸气活动。从包括梨状皮质和杏仁核的边缘和旁边缘区域的喙切割表面检测到自发振荡爆发活动（高达10/min）。爆发活动最初出现在梨状皮质，然后传播到杏仁核。我们使用C4吸气活动作为触发信号，对边缘区的成像数据进行平均。结果表明，在梨状核-杏仁核复合体中的节律性爆发活动与延髓吸气活动的功能耦合，这些信号的互相关分析证实了这一点。这种有节奏的爆发活动可能与处理嗅觉、情感和呼吸相关信息的神经元回路的发展有关。
The amygdala is important for the formation of emotions that are affected by olfactory information. The piriform cortex is involved in information processing related to olfaction. To investigate functional interactions between the piriform cortex and amygdala and their relation to medullary respiratory activity, we developed a novel in vitro preparation including the limbic system, brainstem, and spinal cord of newborn rats. With the use of optical and electrophysiologic recordings, we analyzed spontaneous neuronal activity in the piriform–amygdala complex in limbic–brainstem–spinal cord preparations from 0- to 1-day-old rats. For optical recordings, the preparation was stained with a voltage-sensitive dye, and inspiratory activity was monitored from the fourth cervical (C4) ventral root. Spontaneous oscillatory burst activity (up to 10/min) was detected from the rostral cut surface of limbic and para-limbic regions including the piriform cortex and amygdala. The burst activity initially appeared in the piriform cortex and then propagated to the amygdala. We averaged the imaging data in the limbic area with the use of C4 inspiratory activity as a trigger signal. The results suggest functional coupling of the rhythmic burst activity in the piriform–amygdala complex to medullary inspiratory activity, which was confirmed electrophysiologically by cross-correlation analysis of these signals. This rhythmic burst activity may be involved in the development of neuronal circuits that process information related to olfaction, emotion, and respiration.