Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities.
Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities.
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DOI：
10.1038/ncomms8581
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发表时间：
2015-07-03
影响因子：
16.6
通讯作者：
Croquette V
中科院分区：
文献类型：
--
作者：
Fiorini F;Bagchi D;Le Hir H;Croquette V
RNA解旋酶参与大多数细胞RNA依赖性事件。然而，在真核生物中，只有很少的功能特征。Upf1是一种RNA解旋酶，对无义介导的mRNA衰变（NMD）至关重要。在这里，使用磁镊和批量测定，我们观察到，人类Upf 1能够在长单链核酸上缓慢移位，持续合成能力>10 kb。Upf1通过双链结构和蛋白结合序列有效地易位，表明Upf1是一种有效的核糖核蛋白复合物重塑剂。我们对真核RNA解旋酶进行性解旋的观察表明，Upf 1一旦被募集到NMD mRNA靶点上，就可以扫描整个转录本，以不可逆地重塑mRNP，促进其被NMD机制降解。 Upf1是一种多功能解旋酶，参与各种DNA和RNA相关过程，包括无义介导的mRNA衰变（NMD）。在这里，作者证明了Upf1是一种高度加工性的核糖核蛋白复合物重塑剂，这种能力对Upf1的NMD功能可能很重要。
RNA helicases are implicated in most cellular RNA-dependent events. In eukaryotes however, only few have been functionally characterized. Upf1 is a RNA helicase essential for nonsense-mediated mRNA decay (NMD). Here, using magnetic tweezers and bulk assays, we observe that human Upf1 is able to translocate slowly over long single-stranded nucleic acids with a processivity >10 kb. Upf1 efficiently translocates through double-stranded structures and protein-bound sequences, demonstrating that Upf1 is an efficient ribonucleoprotein complex remodeler. Our observation of processive unwinding by an eukaryotic RNA helicase reveals that Upf1, once recruited onto NMD mRNA targets, can scan the entire transcript to irreversibly remodel the mRNP, facilitating its degradation by the NMD machinery. Upf1 is a multifunctional helicase involved in various DNA- and RNA-related processes, including nonsense-mediated mRNA decay (NMD). Here the authors demonstrate that Upf1 is a highly processive ribonucleoprotein complex remodeler—a capability likely important for Upf1's NMD function.