CircRNA Profiling Reveals CircPPARγ Modulates Adipogenic Differentiation via Sponging miR-92a-3p.
CircRNA Profiling Reveals CircPPARγ Modulates Adipogenic Differentiation via Sponging miR-92a-3p.
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DOI：
10.1021/acs.jafc.2c01815
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发表时间：
2022-05
影响因子：
6.1
通讯作者：
Jiyao Wu;Shaoli Zhang;Binglin Yue;Sihuan Zhang;E. Jiang;Hong Chen;X. Lan
中科院分区：
文献类型：
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作者：
Jiyao Wu;Shaoli Zhang;Binglin Yue;Sihuan Zhang;E. Jiang;Hong Chen;X. Lan
脂肪发生描述了从原代脂肪细胞到成熟脂肪细胞的增殖、分化和凋亡，并受到转录后修饰的调节。环状RNA（circRNA）在哺乳动物的发育和生理中起着重要作用。然而，circRNA介导的脂肪形成调控仍然知之甚少。我们分析了牛原代脂肪形成过程中circRNA的表达，检测了16种circRNA候选物，包括脂肪组织中丰富的circPPARγ。过表达（过表达质粒）和干扰使用EdU技术分析牛原代脂肪细胞中小干扰RNA与circPPARγ的结合，以及增殖、分化和凋亡。（5-乙炔基-2 '-脱氧尿苷）细胞增殖、细胞计数试剂盒-8、流式细胞术、TdT介导的dUTP缺口末端标记凋亡测定、油红O染色、定量实时PCR和蛋白质印迹测定，这表明circPPARγ促进脂肪细胞分化并抑制增殖和凋亡。双荧光素酶报告基因分析和RNA免疫沉淀分析表明，circPPARγ与miR-92 a-3 p和YinYang 1（YY 1）结合。揭示了一种新的调节脂肪生成和脂肪沉积的调节途径。
Adipogenesis describes the proliferation, differentiation, and apoptosis of mature adipocytes from primary adipocytes and is regulated by post-transcriptional modifications. Circular RNAs (circRNAs) play critical roles in mammalian development and physiology. However, the circRNA-mediated regulation of adipogenesis remains poorly understood. We profiled circRNA expression during bovine primary adipogenesis, detecting 16 circRNA candidates, including circPPARγ, which was abundant in the adipose tissue. Overexpression (overexpression plasmids) and interference (small interfering RNAs) with circPPARγ in bovine primary adipocytes, and proliferation, differentiation, and apoptosis were analyzed using EdU (5-ethynyl-2'-deoxyuridine) cell proliferation, cell counting kit-8, flow cytometry, TdT-mediated dUTP nick-end labeling apoptosis assay, Oil Red O staining, quantitative real-time PCR, and western blotting assays, which showed that circPPARγ facilitates adipocyte differentiation and inhibits proliferation and apoptosis. Dual-luciferase reporter assay and RNA immunoprecipitation assays indicated that circPPARγ binds miR-92a-3p and YinYang 1 (YY1). A novel regulatory pathway regulating adipogenesis and adipose deposition was revealed.