Kinetics of Th17-related cytokine expression in experimentally induced rat periapical lesions
Kinetics of Th17-related cytokine expression in experimentally induced rat periapical lesions
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实验诱导的大鼠根尖周病变中 Th17 相关细胞因子表达的动力学
DOI：
10.1111/j.1747-4477.2012.00371.x
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发表时间：
2012
期刊：
影响因子：
1.6
通讯作者：
H Suda
中科院分区：
文献类型：
--
作者：
S Wei;N Kawashima;N Suzuki;J Xu;S Takahashi;M Zhou;Y Koizumi;H Suda
Th 17相关细胞因子是各种病理状态中的重要因素，包括炎性骨破坏。本研究探讨了Th 17相关细胞因子对实验诱导的大鼠根尖周病变进展的贡献。根尖周病变是通过暴露下第一磨牙的髓室而诱发的。在病变中观察到多种免疫活性细胞，包括CD 68+巨噬细胞、Ia抗原+细胞和TCRαβ+T细胞。与正常根尖周组织相比，Th 17相关细胞因子IL-17和IL-23以及促炎细胞因子IL-1β和IL-6的表达水平在第14天（扩张期）显著增加。Foxp 3（一种调节性T细胞（Treg）相关基因）和IL-10（一种抗炎细胞因子）的表达水平在28天（慢性期）高于14天。这些发现表明，Th 17相关细胞因子可能是根尖周骨质破坏的主要原因，并且病变扩展可能受抗炎介质的调节。
Th17‐related cytokines are essential factors in various pathological states, including inflammatory bone destruction. This study investigated the contribution of Th17‐related cytokines to the progress of experimentally induced rat periapical lesions. Periapical pathoses were induced by unsealed exposure of the pulp chamber of the lower first molars. A variety of immunocompetent cells, including CD68+macrophages, Ia antigen+cells and TCRαβ+T cells, were observed in the lesions. The expression levels of Th17‐related cytokines,IL‐17andIL‐23, and of pro‐inflammatory cytokines,IL‐1βandIL‐6, were significantly increased at 14 days (expansion stage) compared with normal periapical tissues. The expression levels ofFoxp3, a regulatory T cell (Treg)‐related gene, and ofIL‐10, an anti‐inflammatory cytokine, were higher at 28 days (chronic stage) than at 14 days. These findings suggest that Th17‐related cytokines may be primary contributors to the initiation of periapical bone destruction, and that lesion expansion may be regulated by anti‐inflammatory mediators.