Genome-Wide Analysis of Alternative Splicing in Zea mays during Maize Iranian Mosaic Virus Infection
Genome-Wide Analysis of Alternative Splicing in Zea mays during Maize Iranian Mosaic Virus Infection
复制标题
DOI：
10.1007/s11105-019-01169-y
复制
发表时间：
2019-10-21
影响因子：
2.1
通讯作者：
Dietzgen, Ralf G.
中科院分区：
文献类型：
--
作者：
Ghorbani, Abozar;Tahmasebi, Ahmad;Dietzgen, Ralf G.
玉米伊朗花叶病毒（MIMV）是伊朗重要的核弹状病毒，侵染多种禾本科植物。玉米在转录水平上对MIMV感染有应答。选择性剪接（AS）是一种从单个前体mRNA产生多个mRNA的机制，通常编码具有功能差异的蛋白质同种型。我们进行了全基因组分析的AS响应玉米幼苗中的MIMV，并确定了参与这种分子反应的基因。我们研究的AS事件包括跳过的外显子、选择性3 '剪接位点、选择性5 '剪接位点、互斥外显子和保留内含子。总共有10，881个玉米基因表现出AS，其中601个基因涉及对MIMV感染的反应，186个基因仅在未感染的玉米中发现。AS在一些参与抗病或致病途径的基因中被鉴定。我们证明，在MIMV感染玉米，寄主基因，参与症状的发展，病毒增殖，抗病性和寄主-病原体相互作用的AS机制。基因网络分析表明，10个基因代表了玉米蛋白质网络的枢纽，它们参与响应病原菌的攻击，包括26 S蛋白酶体，14-3-3-like蛋白A，Rop家族，丝裂原活化蛋白激酶，泛素和丝氨酸/苏氨酸蛋白激酶。总之，我们表明，AS发生在玉米响应MIMV感染的转录调控机制，我们确定了在致病性途径中具有关键作用的基因，这些基因在受感染的幼苗中被差异剪接。
Maize Iranian mosaic virus (MIMV) infects several gramineous plants and is an economically important nucleorhabdovirus in Iran. Maize responds to MIMV infection at the transcriptional level. Alternative splicing (AS) is a mechanism that generates multiple mRNAs from a single pre-mRNA, often encoding protein isoforms with functional differences. We carried out genome-wide analysis of AS responses to MIMV in maize seedlings and identified genes involved in this molecular response. The AS events we investigated included skipped exons, alternative 3 ' splice site, alternative 5 ' splice site, mutually exclusive exons, and retained introns. In total 10,881 maize genes showed AS, of which 601 genes were involved in response to MIMV-infection and 186 were found only in uninfected maize. AS was identified in some of the genes that are involved in disease resistance or pathogenicity pathways. We demonstrated that in MIMV-infects maize, host genes that are involved in symptom development, virus multiplication, resistance to pathogens and host-pathogen interaction are affected by AS mechanism. Gene network analysis showed that ten genes represent the hubs for the protein network in maize and that they are involved in response to pathogen attack and include 26S proteasome, 14-3-3-like protein A, Rop family, mitogen-activated protein kinase, ubiquitin and serine/threonine-protein kinases. In conclusion, we showed that AS occurs as a transcriptional regulatory mechanism in maize response to MIMV infection and we identified genes that have the key roles in pathogenicity pathways that were differentially spliced in infected seedlings.