Regulatory blueprint for a chordate embryo
Regulatory blueprint for a chordate embryo
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DOI：
10.1126/science.1123404
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发表时间：
2006-05-26
期刊：
影响因子：
56.9
通讯作者：
Satou, Yutaka
中科院分区：
文献类型：
--
作者：
Imai, Kaoru S.;Levine, Michael;Satou, Yutaka
玻璃海鞘是一个新兴的模式系统，用于阐明发育过程中的基因网络。全面的原位杂交实验已经鉴定出76个调控基因，它们在早期胚胎中具有局部表达模式，此时未分化的卵裂球已确定遵循特定的细胞命运。系统性的基因破坏实验在突变体背景下提供了3000多种基因表达谱的组合。描述幼虫组织形成的推导基因电路图通过计算进行了可视化。这些图构成了玻璃海鞘胚胎的蓝图，并为理解脊索动物身体结构的进化起源提供了基础。
Ciona is an emerging model system for elucidating gene networks in development. Comprehensive in situ hybridization assays have identified 76 regulatory genes with localized expression patterns in the early embryo, at the time when naive blastomeres are determined to follow specific cell fates. Systematic gene disruption assays provided more than 3000 combinations of gene expression profiles in mutant backgrounds. Deduced gene circuit diagrams describing the formation of larval tissues were computationally visualized. These diagrams constitute a blueprint for the Ciona embryo and provide a foundation for understanding the evolutionary origins of the chordate body plan.