Rapid and efficient detection of transgene homozygosity by FISH of mouse fibroblasts
Rapid and efficient detection of transgene homozygosity by FISH of mouse fibroblasts
复制标题
DOI：
10.1007/s00335-001-2128-5
复制
发表时间：
2002-04-01
期刊：
影响因子：
2.5
通讯作者：
Lamb, BT
中科院分区：
文献类型：
--
作者：
Kulnane, LS;Lehman, EJH;Lamb, BT
本文主要研究FISH技术快速、高效检测小鼠成纤维细胞转基因纯合性。纯合子转基因小鼠的产生在现代生物学研究中有许多非常有用的应用。首先，与相应的半合子状态相比，纯合子转基因通常表达水平更高，并经常导致表型改变。已经发展了几种方法来在半合子动物之间杂交的后代中鉴定纯合子转基因小鼠。
This article focuses on a study related to rapid and efficient detection of transgene homozygosity by FISH of mouse fibroblasts. The generation of homozygous transgenic mice has a number of extremely useful applications in modern biological research. First, a homozygous transgene is generally expressed at a higher level when compared with the corresponding hemizygous state and often leads to altered phenotypes. Several methods have been developed for the identification of homozygous transgenic mice among the offspring from crosses between hemizygous animals.