Circadian regulation of muscle growth independent of locomotor activity.
Circadian regulation of muscle growth independent of locomotor activity.
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DOI：
10.1073/pnas.2012450117
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发表时间：
2020-12-08
影响因子：
11.1
通讯作者：
Hughes SM
中科院分区：
文献类型：
--
作者：
Kelu JJ;Pipalia TG;Hughes SM
一个长期存在的问题，特别是在物理治疗和运动医学中，是一天中的时间是否会影响肌肉代谢，从而影响肌肉的生长，无论是内在的还是对运动或营养的反应。答案将有助于确定一天中锻炼、锻炼肌肉和预防与衰老或疾病相关的肌肉减少症的最佳时间。在这里，我们解决这个问题，在活的斑马鱼肌节在体内，没有其他昼夜节律振荡，如自发活动和食物摄入的干扰。我们发现，活跃的肌肉在白天合成代谢更多，生长更快，而在晚上分解代谢更多，生长更慢。这种昼夜差异在不活动的肌肉中仍然存在，但在生物钟中断后消失。我们的结论是，肌肉显示昼夜节律的差异，独立的活动和喂养的增长。肌肉组织在功能、生理和新陈代谢方面表现出昼夜变化。这种变化是否取决于生物钟本身或继发于身体活动和进食模式的昼夜差异尚不清楚。通过测量肌肉生长超过12小时的时间在活的预喂养的幼体斑马鱼，我们表明，肌肉生长在白天比晚上。显性负性时钟（ΔCLK）的表达可抑制分子时钟功能，消除昼夜节律差异并降低肌肉生长。抑制肌肉收缩会降低白天和夜晚的生长，但不会消除昼夜差异。与夜间相比，昼夜节律钟和身体活动都需要促进白天更高的肌肉蛋白质合成，而蛋白质降解的标志物murf信使RNA在夜间更高。蛋白酶体抑制剂在夜间增加肌肉生长，与体力活动无关，但在白天没有影响。尽管体力活动增强了TORC1的活性，而TORC1抑制剂雷帕霉素抑制了生物钟驱动的白天生长，但没有检测到对夜间肌肉生长的影响。重要的是，1）肌肉生长，2）蛋白质合成和3）murf表达的昼夜差异在自由运行的恒定条件下在夹带的幼虫中都持续存在，表明昼夜节律驱动。通过暴露于永久的黑暗或光照来去除昼夜节律输入导致肌肉生长不佳。我们的结论是，肌肉生长和代谢的昼夜变化是一个昼夜节律的属性，是独立的，但增强，体力活动，至少在发展过程中。
A long-standing question, particularly in physiotherapy and sports medicine, is whether time of day affects muscle metabolism and hence growth, either intrinsically or in response to exercise or nutrition. Answers would help to identify the best time of day to exercise, build muscle, and prevent aging- or disease-related sarcopenia. Here, we address this question in live zebrafish myotome in vivo, without interference from other circadian oscillations such as locomotor activity and food intake. We show that active muscle anabolizes more in the day and grows faster, while catabolizing more at night and growing slower. Such day/night differences remain in inactive muscle but disappear after clock disruption. We conclude that muscles display circadian differences in growth independent of activity and feeding. Muscle tissue shows diurnal variations in function, physiology, and metabolism. Whether such variations are dependent on the circadian clock per se or are secondary to circadian differences in physical activity and feeding pattern is unclear. By measuring muscle growth over 12-h periods in live prefeeding larval zebrafish, we show that muscle grows more during day than night. Expression of dominant negative CLOCK (ΔCLK), which inhibits molecular clock function, ablates circadian differences and reduces muscle growth. Inhibition of muscle contraction reduces growth in both day and night, but does not ablate the day/night difference. The circadian clock and physical activity are both required to promote higher muscle protein synthesis during the day compared to night, whereas markers of protein degradation, murf messenger RNAs, are higher at night. Proteasomal inhibitors increase muscle growth at night, irrespective of physical activity, but have no effect during the day. Although physical activity enhances TORC1 activity, and the TORC1 inhibitor rapamycin inhibits clock-driven daytime growth, no effect on muscle growth at night was detected. Importantly, day/night differences in 1) muscle growth, 2) protein synthesis, and 3) murf expression all persist in entrained larvae under free-running constant conditions, indicating circadian drive. Removal of circadian input by exposure to either permanent darkness or light leads to suboptimal muscle growth. We conclude that diurnal variations in muscle growth and metabolism are a circadian property that is independent of, but augmented by, physical activity, at least during development.
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影响因子：
8.1
作者：
Dyar KA;Ciciliot S;Wright LE;Biensø RS;Tagliazucchi GM;Patel VR;Forcato M;Paz MI;Gudiksen A;Solagna F;Albiero M;Moretti I;Eckel-Mahan KL;Baldi P;Sassone-Corsi P;Rizzuto R;Bicciato S;Pilegaard H;Blaauw B;Schiaffino S
通讯作者：
Schiaffino S
影响因子：
4.1
作者：
DAHL, SV;HALLBRUCKER, C;HAUSSINGER, D
通讯作者：
HAUSSINGER, D
昼夜主义代谢的地图集揭示了时钟之间全系统的协调和通信。
影响因子：
64.5
作者：
Dyar KA;Lutter D;Artati A;Ceglia NJ;Liu Y;Armenta D;Jastroch M;Schneider S;de Mateo S;Cervantes M;Abbondante S;Tognini P;Orozco-Solis R;Kinouchi K;Wang C;Swerdloff R;Nadeef S;Masri S;Magistretti P;Orlando V;Borrelli E;Uhlenhaut NH;Baldi P;Adamski J;Tschöp MH;Eckel-Mahan K;Sassone-Corsi P
通讯作者：
Sassone-Corsi P
DOI：
10.1007/s00335-016-9643-x
发表时间：
2016-08
期刊：
Mammalian genome : official journal of the International Mammalian Genome Society
影响因子：
--
作者：
Goljanek-Whysall K;Iwanejko LA;Vasilaki A;Pekovic-Vaughan V;McDonagh B
通讯作者：
McDonagh B
DOI：
10.1073/pnas.1014523107
发表时间：
2010-11-02
影响因子：
11.1
作者：
Andrews, Jessica L.;Zhang, Xiping;Esser, Karyn A.
通讯作者：
Esser, Karyn A.