Elevation in pulmonary neutrophils and prolonged production of pulmonary macrophage inflammatory protein-2 after burn injury with prior alcohol exposure
Elevation in pulmonary neutrophils and prolonged production of pulmonary macrophage inflammatory protein-2 after burn injury with prior alcohol exposure
复制标题
DOI：
10.1165/ajrcmb.20.6.3491
复制
发表时间：
1999-06-01
影响因子：
6.4
通讯作者：
Kovacs, EJ
中科院分区：
文献类型：
--
作者：
Patel, PJ;Faunce, DE;Kovacs, EJ
各种研究表明，在热损伤之前暴露于酒精会导致发病率和死亡率增加。肺功能衰竭是这些患者的主要并发症，本研究探讨了酒精暴露对烧伤后肺病理学的影响。有一个显着的增加，中性粒细胞募集在肺热损伤后，在这里，我们表明，这似乎是显着升高，在烧伤前给予酒精的动物。因此，我们选择确定是否有差异的巨噬细胞炎性蛋白（MIP）-2，一种有效的中性粒细胞化学引诱物，在小鼠中受到15%的总体表面积的创伤（或假）损伤，有或没有事先乙醇治疗。在损伤后的不同时间点获得肺组织，并通过酶联免疫吸附测定法测定匀浆中的MIP-2。在2小时后，烧伤和烧伤+酒精治疗的小鼠产生的趋化因子的峰值水平。这表示比基线增加了7倍。在单独暴露于烧伤的小鼠中，MIP-2水平在8小时内恢复到基线水平。相比之下，在烧伤前给予酒精的小鼠在8小时继续显示趋化因子水平升高，之后MIP-2下降。这项研究可能提供了一个基础，了解机制负责增加中性粒细胞存在于肺热损伤后，在个人谁消耗酒精。随后，这可能导致在这些患者中观察到的增强的嗜中性粒细胞介导的肺损伤。
Various studies have shown that alcohol exposure before thermal injury leads to increased morbidity and mortality. Pulmonary failure is a major complication seen in these patients, This study examines the effects of prior alcohol exposure on lung pathology after burn injury. There is a marked increase in neutrophil recruitment in the lung after thermal injury, and herein we show that this appears to be significantly elevated in animals given alcohol before burn injury. Consequently, we chose to determine whether there is a difference in pulmonary production of macrophage inflammatory protein (MIP)-2, a potent neutrophil chemoattractant, in mice subjected to a 15% total body surface area scald (or sham) injury with or without prior ethanol treatment. Lung tissue was obtained at various time points after injury and homogenates were assayed for MIP-2 by enzyme-linked immunosorbent assay. At 2 h after injury, peak levels of the chemokine were produced in both burn and burn + alcohol-treated mice. This represents a 7-fold increase above baseline. In mice exposed to burn injury alone, the level of MIP-2 returned to baseline within 8 h. In contrast, mice given alcohol before burn injury continued to show elevated levels of the chemokine at 8 h, after which MIP-2 decreased. This study may provide a basis for understanding the mechanism responsible for the increased neutrophil presence in the lung after thermal injury in individuals who have consumed alcohol. Subsequently, this may lead to the enhanced neutrophil-mediated pulmonary damage observed in these patients.