Microfluidic cytometric analysis of cancer cell transportability and invasiveness.
Microfluidic cytometric analysis of cancer cell transportability and invasiveness.
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DOI：
10.1038/srep14272
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发表时间：
2015-09-25
影响因子：
4.6
通讯作者：
Qin L
中科院分区：
文献类型：
--
作者：
Liu Z;Lee Y;Jang Jh;Li Y;Han X;Yokoi K;Ferrari M;Zhou L;Qin L
癌细胞广泛的表型和功能异质性在肿瘤进展和治疗抗性中起重要作用。表征这种异质性并鉴定侵袭性表型可能为改善化疗治疗提供可能性。通过模拟癌细胞在转移中通过循环系统的灌注，我们开发了一种独特的微流控细胞术（MC）平台，以高通量分离癌细胞，并进一步推导出物理参数“可输送性”来表征通过微收缩的能力。可转运性由细胞硬度和细胞表面摩擦性质决定，并且可用于探测肿瘤异质性，区分更具侵袭性的表型，并与乳腺癌细胞中的生物标志物表达相关。细胞刚度和细胞表面摩擦力的降低导致可转运性的增加，并且可能是通过促进细胞灌注通过循环系统中的狭窄空间的侵袭性癌细胞的特征。微流控芯片为研究细胞力学和可转运性提供了一个有前途的微流控平台，可用作探测肿瘤异质性和确定侵袭表型的新标记物。
The extensive phenotypic and functional heterogeneity of cancer cells plays an important role in tumor progression and therapeutic resistance. Characterizing this heterogeneity and identifying invasive phenotype may provide possibility to improve chemotherapy treatment. By mimicking cancer cell perfusion through circulatory system in metastasis, we develop a unique microfluidic cytometry (MC) platform to separate cancer cells at high throughput, and further derive a physical parameter ‘transportability’ to characterize the ability to pass through micro-constrictions. The transportability is determined by cell stiffness and cell-surface frictional property, and can be used to probe tumor heterogeneity, discriminate more invasive phenotypes and correlate with biomarker expressions in breast cancer cells. Decreased cell stiffness and cell-surface frictional force leads to an increase in transportability and may be a feature of invasive cancer cells by promoting cell perfusion through narrow spaces in circulatory system. The MC-Chip provides a promising microfluidic platform for studying cell mechanics and transportability could be used as a novel marker for probing tumor heterogeneity and determining invasive phenotypes.